Articles by Sharon Lam in JoVE

Expanding Cytotoxic T Lymphocytes from Umbilical Cord Blood that Target Cytomegalovirus, Epstein-Barr Virus, and Adenovirus

JoVE 3627 5/07/2012

Patrick J. Hanley¹, Sharon Lam^1,2, Elizabeth J. Shpall³, Catherine M. Bollard^1,2,4,5

¹Center for Cell and Gene Therapy, Baylor College of Medicine, ²Pathology and Immunology, Baylor College of Medicine, ³Department of Stem Cell Transplantation and Cellular Therapy, University of Texas M.D. Anderson Cancer Center, ⁴Medicine, Baylor College of Medicine, ⁵Department of Pediatrics, Baylor College of Medicine

Here we describe the first good manufacturing practice (GMP)-compliant method of producing virus-specific cytotoxic T lymphocytes (CTL) from umbilical cord blood, a source of predominantly naîve T cells.