Sophia Hober School of Biotechnology, Department of Proteomics Royal Institute of Technology Biography Publications Institution JoVE Articles Sophia Hober has not added a biography. If you are Sophia Hober and would like to personalize this page please email our Author Liaison for assistance. Publications Covalent Immunoglobulin Labeling Through a Photoactivable Synthetic Z Domain Bioconjugate Chemistry. Dec, 2011 | Pubmed ID: 22026370 Engineering Bispecificity into a Single Albumin-binding Domain PloS One. 2011 | Pubmed ID: 21991353 High Nuclear RBM3 Expression is Associated with an Improved Prognosis in Colorectal Cancer Proteomics. Clinical Applications. Dec, 2011 | Pubmed ID: 21956899 Editorial: Biotech Reviews - Keeping Up with Current Developments Biotechnology Journal. Sep, 2011 | Pubmed ID: 21910253 Translational Database Selection and Multiplexed Sequence Capture for Up Front Filtering of Reliable Breast Cancer Biomarker Candidates PloS One. 2011 | Pubmed ID: 21698250 Parallel Production and Verification of Protein Products Using a Novel High-throughput Screening Method Biotechnology Journal. Aug, 2011 | Pubmed ID: 21681961 Surrogate Antigens As Targets for Proteome-wide Binder Selection New Biotechnology. Jul, 2011 | Pubmed ID: 21232647 Enhancing the Protein Production Levels in Escherichia Coli with a Strong Promoter The FEBS Journal. Mar, 2011 | Pubmed ID: 21205203 Targeted Protein Pullout from Human Tissue Samples Using Competitive Elution Biotechnology Journal. Jan, 2011 | Pubmed ID: 21170982 Editorial: Global Biotech Challenges Biotechnology Journal. Dec, 2010 | Pubmed ID: 21154666 Towards a Knowledge-based Human Protein Atlas Nature Biotechnology. Dec, 2010 | Pubmed ID: 21139605 A Small Bispecific Protein Selected for Orthogonal Affinity Purification Biotechnology Journal. Jun, 2010 | Pubmed ID: 20518064 Affibody Molecule-mediated Depletion of HSA and IgG Using Different Buffer Compositions: a 15 Min Protocol for Parallel Processing of 1-48 Samples Biotechnology and Applied Biochemistry. Jun, 2010 | Pubmed ID: 20446920 The Impact of Tissue Fixatives on Morphology and Antibody-based Protein Profiling in Tissues and Cells The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society. Mar, 2010 | Pubmed ID: 19901271 A Global View of Protein Expression in Human Cells, Tissues, and Organs Molecular Systems Biology. 2009 | Pubmed ID: 20029370 Editorial: Biotech in the Post Genomic Era Biotechnology Journal. Dec, 2009 | Pubmed ID: 20014219 Tissue Profiling of the Mammalian Central Nervous System Using Human Antibody-based Proteomics Molecular & Cellular Proteomics : MCP. Jul, 2009 | Pubmed ID: 19351664 Automated Sample Preparation Method for Mass Spectrometry Analysis on Recombinant Proteins Journal of Chromatography. A. May, 2009 | Pubmed ID: 19345368 High-throughput Protein Production--lessons from Scaling Up from 10 to 288 Recombinant Proteins Per Week Biotechnology Journal. Jan, 2009 | Pubmed ID: 19039781 Generation and Validation of Affinity Reagents on a Proteome-wide Level Journal of Molecular Recognition : JMR. Mar-Apr, 2009 | Pubmed ID: 18546091 Development of Affinity Columns for the Removal of High-abundance Proteins in Cerebrospinal Fluid Biotechnology and Applied Biochemistry. Feb, 2009 | Pubmed ID: 18412540 MAP20, a Microtubule-associated Protein in the Secondary Cell Walls of Hybrid Aspen, is a Target of the Cellulose Synthesis Inhibitor 2,6-dichlorobenzonitrile Plant Physiology. Nov, 2008 | Pubmed ID: 18805954 Evaluation of Monospecific Antibodies: a Comparison Study with Commercial Analogs Using Immunohistochemistry on Tissue Microarrays Applied Immunohistochemistry & Molecular Morphology : AIMM / Official Publication of the Society for Applied Immunohistochemistry. Oct, 2008 | Pubmed ID: 18685494 A Genecentric Human Protein Atlas for Expression Profiles Based on Antibodies Molecular & Cellular Proteomics : MCP. Oct, 2008 | Pubmed ID: 18669619 A Web-based Tool for in Silico Biomarker Discovery Based on Tissue-specific Protein Profiles in Normal and Cancer Tissues Molecular & Cellular Proteomics : MCP. May, 2008 | Pubmed ID: 17913849 Affibody-mediated Transferrin Depletion for Proteomics Applications Biotechnology Journal. Nov, 2007 | Pubmed ID: 17639529 Z(basic)--a Novel Purification Tag for Efficient Protein Recovery Journal of Chromatography. A. Aug, 2007 | Pubmed ID: 17570380 High-throughput Protein Purification Under Denaturating Conditions by the Use of Cation Exchange Chromatography Biotechnology Journal. Jun, 2007 | Pubmed ID: 17492715 Approaches for Systematic Proteome Exploration Biomolecular Engineering. Jun, 2007 | Pubmed ID: 17376740 The RBCC Gene RFP2 (Leu5) Encodes a Novel Transmembrane E3 Ubiquitin Ligase Involved in ERAD Molecular Biology of the Cell. May, 2007 | Pubmed ID: 17314412 Multiplexed PrEST Immunization for High-throughput Affinity Proteomics Journal of Immunological Methods. Aug, 2006 | Pubmed ID: 16949094 Single-step Recovery and Solid-phase Refolding of Inclusion Body Proteins Using a Polycationic Purification Tag Biotechnology Journal. Feb, 2006 | Pubmed ID: 16892247 Microfluidic Analysis of Antibody Specificity in a Compact Disk Format Journal of Proteome Research. Jul, 2006 | Pubmed ID: 16823963 A Human Protein Atlas Based on Antibody Proteomics Current Opinion in Molecular Therapeutics. Jun, 2006 | Pubmed ID: 16774037 From Gene Expression Analysis to Tissue Microarrays: a Rational Approach to Identify Therapeutic and Diagnostic Targets in Lymphoid Malignancies Molecular & Cellular Proteomics : MCP. Jun, 2006 | Pubmed ID: 16524965 High-throughput Protein Purification Using an Automated Set-up for High-yield Affinity Chromatography Protein Expression and Purification. Apr, 2006 | Pubmed ID: 16483795 Pyrosequencing: History, Biochemistry and Future Clinica Chimica Acta; International Journal of Clinical Chemistry. Jan, 2006 | Pubmed ID: 16165119 Towards a Human Proteome Atlas: High-throughput Generation of Mono-specific Antibodies for Tissue Profiling Proteomics. Nov, 2005 | Pubmed ID: 16237735 A Human Protein Atlas for Normal and Cancer Tissues Based on Antibody Proteomics Molecular & Cellular Proteomics : MCP. Dec, 2005 | Pubmed ID: 16127175 High-throughput Solubility Assay for Purified Recombinant Protein Immunogens Biochimica Et Biophysica Acta. Aug, 2005 | Pubmed ID: 16084133 A Novel Flow Cytometry-based Method for Analysis of Expression Levels in Escherichia Coli, Giving Information About Precipitated and Soluble Protein Journal of Biotechnology. Sep, 2005 | Pubmed ID: 15996784 Affinity Ligands for Industrial Protein Purification Protein and Peptide Letters. May, 2005 | Pubmed ID: 15907172 Effect of Substrate Feed Rate on Recombinant Protein Secretion, Degradation and Inclusion Body Formation in Escherichia Coli Applied Microbiology and Biotechnology. Jul, 2005 | Pubmed ID: 15655679 Selective Enrichment of Monospecific Polyclonal Antibodies for Antibody-based Proteomics Efforts Journal of Chromatography. A. Jul, 2004 | Pubmed ID: 15317410 Genome-based Proteomics Electrophoresis. May, 2004 | Pubmed ID: 15174050 Toward Pyrosequencing on Surface-attached Genetic Material by Use of DNA-binding Luciferase Fusion Proteins Analytical Biochemistry. Jun, 2004 | Pubmed ID: 15136162 Improving the Tolerance of a Protein a Analogue to Repeated Alkaline Exposures Using a Bypass Mutagenesis Approach Proteins. May, 2004 | Pubmed ID: 15048831 Evaluation of Different Linker Regions for Multimerization and Coupling Chemistry for Immobilization of a Proteinaceous Affinity Ligand Protein Engineering. Dec, 2003 | Pubmed ID: 14983098 An Improved Dual-expression Concept, Generating High-quality Antibodies for Proteomics Research Biotechnology and Applied Biochemistry. Dec, 2003 | Pubmed ID: 12875650 Methylotrophic Yeast Pichia Pastoris As a Host for Production of ATP-diphosphohydrolase (apyrase) from Potato Tubers (Solanum Tuberosum) Protein Expression and Purification. Feb, 2003 | Pubmed ID: 12597881 Engineering Streptococcal Protein G for Increased Alkaline Stability Protein Engineering. Oct, 2002 | Pubmed ID: 12468718 Escherichia Coli Single-stranded DNA-binding Protein, a Molecular Tool for Improved Sequence Quality in Pyrosequencing Electrophoresis. Sep, 2002 | Pubmed ID: 12373756 Integrated Strategy for Selective Expanded Bed Ion-exchange Adsorption and Site-specific Protein Processing Using Gene Fusion Technology Journal of Biotechnology. Jun, 2002 | Pubmed ID: 12142146 Strategy for Highly Selective Ion-exchange Capture Using a Charge-polarizing Fusion Partner Journal of Chromatography. A. Jan, 2002 | Pubmed ID: 11822381 Mutational Analysis of the Interaction Between Albumin-binding Domain from Streptococcal Protein G and Human Serum Albumin Protein Science : a Publication of the Protein Society. Feb, 2002 | Pubmed ID: 11790830 Structure, Specificity, and Mode of Interaction for Bacterial Albumin-binding Modules The Journal of Biological Chemistry. Mar, 2002 | Pubmed ID: 11751858 Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag Johan Nilvebrant1, Tove Alm1, Sophia Hober1 1School of Biotechnology, Department of Proteomics, Royal Institute of Technology JoVE 3370 Biology
Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag Johan Nilvebrant1, Tove Alm1, Sophia Hober1 1School of Biotechnology, Department of Proteomics, Royal Institute of Technology JoVE 3370 Biology