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In JoVE (1)
Other Publications (1)
Articles by Srividya Chandramouli Iyer in JoVE
Isolation and Purification of Drosophila Peripheral Neurons by Magnetic Bead Sorting
Eswar Prasad R. Iyer1,2, Srividya Chandramouli Iyer1,2, Mikolaj J. Sulkowski1,2, Daniel N. Cox1,2
1Department of Molecular and Microbiology, George Mason University, 2Krasnow Institute for Advanced Study, George Mason University
In this video-article we present a method for the isolation and purification of Drosophila peripheral neurons using a fast magnetic bead assisted cell sorting strategy. RNA obtained from the isolated cells can be readily used for downstream applications including microarray analyses.
Other articles by Srividya Chandramouli Iyer on PubMed
Turtle Functions Downstream of Cut in Differentially Regulating Class Specific Dendrite Morphogenesis in Drosophila
PloS One. 2011 | Pubmed ID: 21811639
Dendritic morphology largely determines patterns of synaptic connectivity and electrochemical properties of a neuron. Neurons display a myriad diversity of dendritic geometries which serve as a basis for functional classification. Several types of molecules have recently been identified which regulate dendrite morphology by acting at the levels of transcriptional regulation, direct interactions with the cytoskeleton and organelles, and cell surface interactions. Although there has been substantial progress in understanding the molecular mechanisms of dendrite morphogenesis, the specification of class-specific dendritic arbors remains largely unexplained. Furthermore, the presence of numerous regulators suggests that they must work in concert. However, presently, few genetic pathways regulating dendrite development have been defined.