Articles by Stephanie Bacopulos in JoVE

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR (qPCR)

JoVE 3874 5/16/2012

Aida Gordanpour¹, Robert K. Nam², Linda Sugar³, Stephanie Bacopulos¹, Arun Seth^1,3,4

¹Department of Laboratory Medicine & Pathobiology, University of Toronto, ²Division of Urology, Sunnybrook Health Sciences Centre, Toronto, Canada, ³Department of Anatomic Pathology, Sunnybrook Health Sciences Centre, Toronto, Canada, ⁴Biological Sciences, Sunnybrook Research Institute

Quantitative Real Time polymerase chain reaction (qPCR) is a rapid and sensitive method to investigate the expression levels of various microRNA (miRNA) molecules in tumor samples. Using this method expression of hundreds of different miRNA molecules can be amplified, quantified, and analyzed from the same cDNA template.