Articles by Tiffany Heiple in JoVE

Staining Protocols for Human Pancreatic Islets

JoVE 4068 5/23/2012

Martha L. Campbell-Thompson, Tiffany Heiple, Emily Montgomery, Li Zhang, Lynda Schneider

Department of Pathology, Immunology, and Laboratory Medicine, University of Florida

This video demonstrates procedures for characterization of human pancreatic islets using hematoxylin and eosin (H&E) and immunohistochemistry (IHC). Pancreatic sections from head, body, and tail regions are stained by both H&E and IHC to determine islet endocrine composition (insulin, glucagon, and pancreatic polypeptide), cell replication (Ki67), and inflammatory infiltrates (H&E, CD3). The uncinate region is localized using IHC for pancreatic polypeptide.