Articles by Tyson J. Edwards in JoVE

In vivo Laser Axotomy in C. elegans

JoVE 2707 5/19/2011

Alexandra B. Byrne*, Tyson J. Edwards*, Marc Hammarlund

Department of Genetics, Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine

A protocol to cut neurons in C. elegans with a MicroPoint pulsed laser is presented. We describe setting up the system, immobilizing worms, and severing labeled neurons. Advantages include a relatively low-cost system and the ability to sever neuronal processes or ablate cells in vivo.