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In JoVE (1)
Other Publications (2)
Articles by Uihan Kim in JoVE
A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering
Uihan Kim1, Jaewoo Song2, Suho Ryu1, Soocheol Kim1, Chulmin Joo1
1School of Mechanical Engineering, Yonsei University, 2Department of Laboratory Medicine, Yonsei University
Other articles by Uihan Kim on PubMed
Capillary-scale Direct Measurement of Hemoglobin Concentration of Erythrocytes Using Photothermal Angular Light Scattering
Biosensors & Bioelectronics. Dec, 2015 | Pubmed ID: 26176206
We present a direct, rapid and chemical-free detection method for hemoglobin concentration ([Hb]), based on photothermal angular light scattering. The iron oxides contained in hemoglobin molecules exhibit high absorption of 532-nm light and generate heat under the illumination of 532-nm light, which subsequently alters the refractive index of blood. We measured this photothermal change in refractive index by employing angular light scattering spectroscopy with the goal of quantifying [Hb] in blood samples. Highly sensitive [Hb] measurement of blood samples was performed by monitoring the shifts in angularly dispersed scattering patterns from the blood-loaded microcapillary tubes. Our system measured [Hb] over the range of 0.35-17.9 g/dL with a detection limit of ~0.12 g/dL. Our sensor was characterized by excellent correlation with a reference hematology analyzer (r>0.96), and yielded a precision of 0.63 g/dL for a blood sample of 9.0 g/dL.
Biomedical Optics Express. Dec, 2015 | Pubmed ID: 26713205
We present a multi-contrast microscope based on color-coded illumination and computation. A programmable three-color light-emitting diode (LED) array illuminates a specimen, in which each color corresponds to a different illumination angle. A single color image sensor records light transmitted through the specimen, and images at each color channel are then separated and utilized to obtain bright-field, dark-field, and differential phase contrast (DPC) images simultaneously. Quantitative phase imaging is also achieved based on DPC images acquired with two different LED illumination patterns. The multi-contrast and quantitative phase imaging capabilities of our method are demonstrated by presenting images of various transparent biological samples.