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In JoVE (1)
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Articles by Xavier Casadevall i Solvas in JoVE
JoVE Bioengineering
Fluorescens detektionsmetoder för mikroflödessystem droppe plattformar
1Department of Chemistry, Imperial College London, 2Department of Biochemistry, Protein Chip Research Center, Chungbuk National University, 3Department of Chemistry and Applied Biosciences, Institute for Chemical and Bioengineering, ETH Zurich
Droplet-baserade mikroflödessystem plattformar är lovande kandidater för hög genomströmning experiment eftersom de kan generera pikoliter, själv-compartmentalized fartyg billigt till kHz priser. Genom integration med snabba, känsliga och hög upplösning fluorescens spektroskopiska metoder, kan stora mängder information som genereras inom dessa system effektivt extraheras, utnyttjas och används.
Other articles by Xavier Casadevall i Solvas on PubMed
Mapping of Fluidic Mixing in Microdroplets with 1 Micros Time Resolution Using Fluorescence Lifetime Imaging
Microdroplets generated in microfluidic channels hold great promise for use as substrates in high-throughput chemical and biological analysis. These water-in-oil compartments can serve as isolated reaction vessels, and since they can be generated at rates in excess of 1 kHz, thousands of assays can be carried out quickly and reproducibly. Nevertheless, sampling the large amount of information generated from these platforms still remains a significant challenge. For example, considering the high droplet generation rates and velocities, reproducibility and micrometer resolution are challenging requirements that must be fulfilled. Herein we combine confocal fluorescence lifetime imaging microscopy with a statistical implementation that permits the analysis of mixing phenomena within microdroplets with a temporal resolution of 1 mus. Importantly, such exquisite resolution is only possible as a result of the large number of droplets sampled and their high structural reproducibility.
Droplet Microfluidics: Recent Developments and Future Applications
We report recent advances in the field of droplet-based microfluidics. Specifically, we highlight the unique features of such platforms for high-throughput experimentation; describe functional components that afford complex analytical processing and report on applications in synthesis, high-throughput screening, cell biology and synthetic and systems biology. Issues including the integration of high-information content detection methods, long term droplet stability and opportunities for large scale and intelligent biological experimentation are also discussed.
High-throughput Age Synchronisation of Caenorhabditis Elegans
We present a passive microfluidic strategy for sorting adult C. elegans nematodes on the basis of age and size. The separation mechanism takes advantage of phenotypic differences between 'adult' and 'juvenile' organisms and their behaviour in microfluidic architectures. In brief, the microfluidic device allows worms to sort themselves in a passive manner.
