Xin-hui Xing Department of Chemical Engineering, Institute of Biochemical Engineering Tsinghua University Biography Publications Institution JoVE Articles Xin-hui Xing has not added a biography. If you are Xin-hui Xing and would like to personalize this page please email our Author Liaison for assistance. Publications Encoding Genetic Circuits with DNA Barcodes Paves the Way for Machine Learning-Assisted Metabolite Biosensor Response Curve Profiling in Yeast ACS Synthetic Biology. Jan, 2022 | Pubmed ID: 35089702 New Method for Genome-Scale Functional Genomic Study in Bacteria with Superior Performance: CRISPR Interference Screen Methods in Molecular Biology (Clifton, N.J.). 2022 | Pubmed ID: 34709614 Advanced Strategies and Tools to Facilitate and Streamline Microbial Adaptive Laboratory Evolution Trends in Biotechnology. Jan, 2022 | Pubmed ID: 33958227 A Versatile Toolbox for CRISPR-based Genome Engineering in Pichia Pastoris Applied Microbiology and Biotechnology. Dec, 2021 | Pubmed ID: 34773154 Binding Ability of Methylene Blue with Heparin Dependent on Its Sulfate Level Rather Than Its Sulfation Location or Basic Saccharide Structure Glycoconjugate Journal. 10, 2021 | Pubmed ID: 34515908 Construction and Characterization of Novel Bifunctional Fusion Proteins Composed of Alcohol Dehydrogenase and NADH Oxidase with Efficient Oxidized Cofactor Regeneration Biotechnology and Applied Biochemistry. Jul, 2021 | Pubmed ID: 34269481 [Functional Discovery and Production Technology for Natural Bioactive Peptides] Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology. Jun, 2021 | Pubmed ID: 34227301 Genome-wide Genotype-phenotype Associations in Microbes Journal of Bioscience and Bioengineering. Jul, 2021 | Pubmed ID: 33895083 [Technology Development and Instrumentation of a High-throughput and Automated Microbial Microdroplet Culture System for Microbial Evolution and Screening] Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology. Mar, 2021 | Pubmed ID: 33783163 Effects of Bioactive Components of Pu-erh Tea on Gut Microbiomes and Health: A Review Food Chemistry. Aug, 2021 | Pubmed ID: 33743430 Metabolomic Analysis Improves Bioconversion of Methanol to Isobutanol in Methylorubrum Extorquens AM1 Biotechnology Journal. Jun, 2021 | Pubmed ID: 33595188 Guide-target Mismatch Effects on DCas9-sgRNA Binding Activity in Living Bacterial Cells Nucleic Acids Research. 02, 2021 | Pubmed ID: 33503261 Rewiring the Native Methanol Assimilation Metabolism by Incorporating the Heterologous Ribulose Monophosphate Cycle into Methylorubrum Extorquens Metabolic Engineering. 03, 2021 | Pubmed ID: 33493644 Design and Construction of Chimeric Linker Library with Controllable Flexibilities for Precision Protein Engineering Methods in Enzymology. 2021 | Pubmed ID: 33482990 A Heparin Derivatives Library Constructed by Chemical Modification and Enzymatic Depolymerization for Exploitation of Non-anticoagulant Functions Carbohydrate Polymers. Dec, 2020 | Pubmed ID: 32933671 Insights into the Molecular-level Effects of Atmospheric and Room-temperature Plasma on Mononucleotides and Single-stranded Homo- and Hetero-oligonucleotides Scientific Reports. 08, 2020 | Pubmed ID: 32868795 Empowering a Methanol-Dependent Via Adaptive Evolution Using a High-Throughput Microbial Microdroplet Culture System Frontiers in Bioengineering and Biotechnology. 2020 | Pubmed ID: 32733857 Increased Stability and Intracellular Antioxidant Activity of Chlorogenic Acid Depend on Its Molecular Interaction with Wheat Gluten Hydrolysate Food Chemistry. Apr, 2020 | Pubmed ID: 32387948 Establishment of CRISPR Interference in Methylorubrum Extorquens and Application of Rapidly Mining a New Phytoene Desaturase Involved in Carotenoid Biosynthesis Applied Microbiology and Biotechnology. May, 2020 | Pubmed ID: 32215707 Microbial Microdroplet Culture System (MMC): An Integrated Platform for Automated, High-throughput Microbial Cultivation and Adaptive Evolution Biotechnology and Bioengineering. 06, 2020 | Pubmed ID: 32159223 Highly Efficient Capture of Marine Microbial Strains in Seawater Using Bare FeO Magnetic Beads Current Microbiology. Jul, 2020 | Pubmed ID: 32103330 Prokaryotic Communities in Multidimensional Bottom-pit-mud from Old and Young Pits Used for the Production of Chinese Strong-Flavor Baijiu Food Chemistry. May, 2020 | Pubmed ID: 31901820 Dynamics of Transcription-translation Coordination Tune Bacterial Indole Signaling Nature Chemical Biology. 04, 2020 | Pubmed ID: 31873224 Novel Breeding Approach for Japanese Flounder Using Atmosphere and Room Temperature Plasma Mutagenesis Tool BMC Genomics. Apr, 2019 | Pubmed ID: 31035925 Genome-wide Screening Identifies Promiscuous Phosphatases Impairing Terpenoid Biosynthesis in Escherichia Coli Applied Microbiology and Biotechnology. Nov, 2018 | Pubmed ID: 30229323 Improved SgRNA Design in Bacteria Via Genome-wide Activity Profiling Nucleic Acids Research. 08, 2018 | Pubmed ID: 29982721 Pooled CRISPR Interference Screening Enables Genome-scale Functional Genomics Study in Bacteria with Superior Performance Nature Communications. 06, 2018 | Pubmed ID: 29946130 Structural Characterization and in Vitro Antioxidant Activities of Chondroitin Sulfate Purified from Andrias Davidianus Cartilage Carbohydrate Polymers. Sep, 2018 | Pubmed ID: 29891311 Massilia Violaceinigra Sp. Nov., a Novel Purple-pigmented Bacterium Isolated from Glacier Permafrost International Journal of Systematic and Evolutionary Microbiology. Jul, 2018 | Pubmed ID: 29851375 Enhanced Production of Crude Violacein from Glucose in Escherichia Coli by Overexpression of Rate-Limiting Key Enzyme(S) Involved in Violacein Biosynthesis Applied Biochemistry and Biotechnology. Dec, 2018 | Pubmed ID: 29797295 Breeding of Methanol-Tolerant Methylobacterium Extorquens AM1 by Atmospheric and Room Temperature Plasma Mutagenesis Combined With Adaptive Laboratory Evolution Biotechnology Journal. Jun, 2018 | Pubmed ID: 29729127 MiYA, an Efficient Machine-learning Workflow in Conjunction with the YeastFab Assembly Strategy for Combinatorial Optimization of Heterologous Metabolic Pathways in Saccharomyces Cerevisiae Metabolic Engineering. 05, 2018 | Pubmed ID: 29627507 Design of Fusion Proteins for Efficient and Soluble Production of Immunogenic Ebola Virus Glycoprotein in Escherichia Coli Biotechnology Journal. Jun, 2018 | Pubmed ID: 29500882 Cre/loxP-Mediated Multicopy Integration of the Mevalonate Operon into the Genome of Methylobacterium Extorquens AM1 Applied Biochemistry and Biotechnology. Jul, 2018 | Pubmed ID: 29243041 A Rapid and Specific Colorimetric Method for Free Tryptophan Quantification Talanta. Jan, 2018 | Pubmed ID: 28917797 Bioprocess Engineering for Biohythane Production from Low-grade Waste Biomass: Technical Challenges Towards Scale Up Current Opinion in Biotechnology. 04, 2018 | Pubmed ID: 28892667 Targeted Mutagenesis: A Sniper-like Diversity Generator in Microbial Engineering Synthetic and Systems Biotechnology. Jun, 2017 | Pubmed ID: 29062964 Effects of Enzymatically Depolymerized Low Molecular Weight Heparins on CCl-Induced Liver Fibrosis Frontiers in Pharmacology. 2017 | Pubmed ID: 28871223 Maltose Utilization As a Novel Selection Strategy for Continuous Evolution of Microbes with Enhanced Metabolite Production ACS Synthetic Biology. 12, 2017 | Pubmed ID: 28841296 Discovery of Enzymatically Depolymerized Heparins Capable of Treating Bleomycin-induced Pulmonary Injury and Fibrosis in Mice Carbohydrate Polymers. Oct, 2017 | Pubmed ID: 28821136 Metabolic Engineering of Escherichia Coli Cell Factory for Highly Active Xanthine Dehydrogenase Production Bioresource Technology. Dec, 2017 | Pubmed ID: 28610971 Erratum To: Continuous Production of Biohythane from Hydrothermal Liquefied Cornstalk Biomass Via Two-stage High-rate Anaerobic Reactors Biotechnology for Biofuels. 2017 | Pubmed ID: 28174602 Non-anticoagulant Effects of Low Molecular Weight Heparins in Inflammatory Disorders: A Review Carbohydrate Polymers. Mar, 2017 | Pubmed ID: 28115102 Biosensor-assisted Transcriptional Regulator Engineering for Methylobacterium Extorquens AM1 to Improve Mevalonate Synthesis by Increasing the Acetyl-CoA Supply Metabolic Engineering. 01, 2017 | Pubmed ID: 27919791 Improved Production of Trans-4-hydroxy-l-proline by Chromosomal Integration of the Vitreoscilla Hemoglobin Gene into Recombinant Escherichia Coli with Expression of Proline-4-hydroxylase Journal of Bioscience and Bioengineering. Jan, 2017 | Pubmed ID: 27567047 Electrochemically-responsive Magnetic Nanoparticles for Reversible Protein Adsorption Journal of Materials Chemistry. B. Jun, 2016 | Pubmed ID: 32263099 Continuous Production of Biohythane from Hydrothermal Liquefied Cornstalk Biomass Via Two-stage High-rate Anaerobic Reactors Biotechnology for Biofuels. 2016 | Pubmed ID: 27895708 Xanthine Dehydrogenase: An Old Enzyme with New Knowledge and Prospects Bioengineered. Nov, 2016 | Pubmed ID: 27537049 Enrichment and Characteristics of Mixed Methane-oxidizing Bacteria from a Chinese Coal Mine Applied Microbiology and Biotechnology. Dec, 2016 | Pubmed ID: 27474022 Enhanced Biohydrogen Production from Corn Stover by the Combination of Clostridium Cellulolyticum and Hydrogen Fermentation Bacteria Journal of Bioscience and Bioengineering. Oct, 2016 | Pubmed ID: 27150511 Establishment of Chondroitin B Lyase-based Analytical Methods for Sensitive and Quantitative Detection of Dermatan Sulfate in Heparin Carbohydrate Polymers. Jun, 2016 | Pubmed ID: 27083825 A Study on the Effects of Linker Flexibility on Acid Phosphatase PhoC-GFP Fusion Protein Using a Novel Linker Library Enzyme and Microbial Technology. Feb, 2016 | Pubmed ID: 26777244 Characterization of a Novel Acinetobacter Baumannii Xanthine Dehydrogenase Expressed in Escherichia Coli Biotechnology Letters. Feb, 2016 | Pubmed ID: 26543035 Bioconversion of Methanol to Value-added Mevalonate by Engineered Methylobacterium Extorquens AM1 Containing an Optimized Mevalonate Pathway Applied Microbiology and Biotechnology. Mar, 2016 | Pubmed ID: 26521242 Intermediate-sensor Assisted Push-pull Strategy and Its Application in Heterologous Deoxyviolacein Production in Escherichia Coli Metabolic Engineering. Jan, 2016 | Pubmed ID: 26506462 Insights into the Global Regulation of Anaerobic Metabolism for Improved Biohydrogen Production Bioresource Technology. Jan, 2016 | Pubmed ID: 26476162 Construction of a Linker Library with Widely Controllable Flexibility for Fusion Protein Design Applied Microbiology and Biotechnology. Jan, 2016 | Pubmed ID: 26394862 Disruption of Lactate Dehydrogenase and Alcohol Dehydrogenase for Increased Hydrogen Production and Its Effect on Metabolic Flux in Enterobacter Aerogenes Bioresource Technology. Oct, 2015 | Pubmed ID: 26188552 Quantitative Evaluation of DNA Damage and Mutation Rate by Atmospheric and Room-temperature Plasma (ARTP) and Conventional Mutagenesis Applied Microbiology and Biotechnology. Jul, 2015 | Pubmed ID: 26025015 DomSign: a Top-down Annotation Pipeline to Enlarge Enzyme Space in the Protein Universe BMC Bioinformatics. Mar, 2015 | Pubmed ID: 25888481 Culture Characteristics of the Atmospheric and Room Temperature Plasma-mutated Spirulina Platensis Mutants in CO2 Aeration Culture System for Biomass Production Journal of Bioscience and Bioengineering. Oct, 2015 | Pubmed ID: 25795571 Performance and Microbial Community of Carbon Nanotube Fixed-bed Microbial Fuel Cell Continuously Fed with Hydrothermal Liquefied Cornstalk Biomass Bioresource Technology. Jun, 2015 | Pubmed ID: 25780905 High Crude Violacein Production from Glucose by Escherichia Coli Engineered with Interactive Control of Tryptophan Pathway and Violacein Biosynthetic Pathway Microbial Cell Factories. Jan, 2015 | Pubmed ID: 25592762 Effects of Furan Derivatives on Biohydrogen Fermentation from Wet Steam-exploded Cornstalk and Its Microbial Community Bioresource Technology. Jan, 2015 | Pubmed ID: 25459816 Hybrid Nanoparticles with CO-responsive Shells and Fluorescence-labelled Magnetic Cores Journal of Materials Chemistry. B. Jan, 2014 | Pubmed ID: 32261388 Atmospheric and Room Temperature Plasma (ARTP) As a New Powerful Mutagenesis Tool Applied Microbiology and Biotechnology. Jun, 2014 | Pubmed ID: 24769904 Controllable Production of Low Molecular Weight Heparins by Combinations of Heparinase I/II/III Carbohydrate Polymers. Jan, 2014 | Pubmed ID: 24299802 Comprehensive Phylogenetic Diversity of [FeFe]-hydrogenase Genes in Termite Gut Microbiota Microbes and Environments. 2013 | Pubmed ID: 24240187 Active Inclusion Bodies of Acid Phosphatase PhoC: Aggregation Induced by GFP Fusion and Activities Modulated by Linker Flexibility Microbial Cell Factories. Mar, 2013 | Pubmed ID: 23497261 Thermostable Alcohol Dehydrogenase from Thermococcus Kodakarensis KOD1 for Enantioselective Bioconversion of Aromatic Secondary Alcohols Applied and Environmental Microbiology. Apr, 2013 | Pubmed ID: 23354700 Rational Design of a Tripartite Fusion Protein of Heparinase I Enables One-step Affinity Purification and Real-time Activity Detection Journal of Biotechnology. Jan, 2013 | Pubmed ID: 23073152 Improvement of Hydrogen Productivity by Introduction of NADH Regeneration Pathway in Clostridium Paraputrificum Applied Biochemistry and Biotechnology. Jun, 2012 | Pubmed ID: 22592776 Combination of Site-directed Mutagenesis and Calcium Ion Addition for Enhanced Production of Thermostable MBP-fused Heparinase I in Recombinant Escherichia Coli Applied Microbiology and Biotechnology. May, 2012 | Pubmed ID: 22588503 Luciferase and Fluorescent Protein As Dual Reporters Analyzing the Effect of N-dodecyltrimethylammonium Bromide on the Physiology of Pseudomonas Putida Applied Microbiology and Biotechnology. Jan, 2012 | Pubmed ID: 22075632 Improved Hydrogen Production Under Microaerophilic Conditions by Overexpression of Polyphosphate Kinase in Enterobacter Aerogenes Enzyme and Microbial Technology. Feb, 2011 | Pubmed ID: 22112830 Enzyme-assisted Extraction of Flavonoids from Ginkgo Biloba Leaves: Improvement Effect of Flavonol Transglycosylation Catalyzed by Penicillium Decumbens Cellulase Enzyme and Microbial Technology. Jan, 2011 | Pubmed ID: 22112777 Bioengineering of the Enterobacter Aerogenes Strain for Biohydrogen Production Bioresource Technology. Sep, 2011 | Pubmed ID: 21764301 Biochemical Analysis and Kinetic Modeling of the Thermal Inactivation of MBP-fused Heparinase I: Implications for a Comprehensive Thermostabilization Strategy Biotechnology and Bioengineering. Aug, 2011 | Pubmed ID: 21445884 Reconstruction of the Violacein Biosynthetic Pathway from Duganella Sp. B2 in Different Heterologous Hosts Applied Microbiology and Biotechnology. Apr, 2010 | Pubmed ID: 20012278 Alteration of Hydrogen Metabolism of Ldh-deleted Enterobacter Aerogenes by Overexpression of NAD+-dependent Formate Dehydrogenase Applied Microbiology and Biotechnology. Mar, 2010 | Pubmed ID: 19830418 Optimization of Effect Factors for Mycelial Growth and Exopolysaccharide Production by Schizophyllum Commune Applied Biochemistry and Biotechnology. Jan, 2010 | Pubmed ID: 19156366 Changes in Biomass Activity and Characteristics of Activated Sludge Exposed to Low Ozone Dose Chemosphere. Sep, 2009 | Pubmed ID: 19698970 Expression of NAD+-dependent Formate Dehydrogenase in Enterobacter Aerogenes and Its Involvement in Anaerobic Metabolism and H2 Production Biotechnology Letters. Oct, 2009 | Pubmed ID: 19533026 Systematic Analysis of Biochemical Performance and the Microbial Community of an Activated Sludge Process Using Ozone-treated Sludge for Sludge Reduction Bioresource Technology. Nov, 2009 | Pubmed ID: 19515555 Diversity and Activity of Methanotrophs in Alkaline Soil from a Chinese Coal Mine FEMS Microbiology Ecology. Nov, 2009 | Pubmed ID: 19515201 Correcting for the Inner Filter Effect in Measurements of Fluorescent Proteins in High-cell-density Cultures Analytical Biochemistry. Jul, 2009 | Pubmed ID: 19394302 Temperature Influence on Fluorescence Intensity and Enzyme Activity of the Fusion Protein of GFP and Hyperthermophilic Xylanase Applied Microbiology and Biotechnology. Sep, 2009 | Pubmed ID: 19390851 Progress and Perspectives of Sludge Ozonation As a Powerful Pretreatment Method for Minimization of Excess Sludge Production Water Research. Apr, 2009 | Pubmed ID: 19282018 Application of Combined Physicochemical and Biological Processes for Enhanced Treatment of Avermectin Fermentation Wastewater Water Science and Technology : a Journal of the International Association on Water Pollution Research. 2009 | Pubmed ID: 19237772 Characteristics of Hydrogen and Methane Production from Cornstalks by an Augmented Two- or Three-stage Anaerobic Fermentation Process Bioresource Technology. Jun, 2009 | Pubmed ID: 19231169 Paraffin Oil As a "methane Vector" for Rapid and High Cell Density Cultivation of Methylosinus Trichosporium OB3b Applied Microbiology and Biotechnology. Jun, 2009 | Pubmed ID: 19219428 Analysis of the Mechanism of Sludge Ozonation by a Combination of Biological and Chemical Approaches Water Research. Jan, 2009 | Pubmed ID: 18976790 Enhanced Coagulation of Ferric Chloride Aided by Tannic Acid for Phosphorus Removal from Wastewater Chemosphere. May, 2008 | Pubmed ID: 18395769 Enhanced Sludge Solubilization by Microbubble Ozonation Chemosphere. May, 2008 | Pubmed ID: 18328535 Enhanced Ozonation of Simulated Dyestuff Wastewater by Microbubbles Chemosphere. Aug, 2007 | Pubmed ID: 17467036 [Study on the Structure and Function of a Stable Methane-oxidizing Mixed Microbial Consortium] Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica. Feb, 2007 | Pubmed ID: 17436634 Functional Expression of the Particulate Methane Mono-oxygenase Gene in Recombinant Rhodococcus Erythropolis FEMS Microbiology Letters. Oct, 2006 | Pubmed ID: 16978347 Biological Wastewater Treatment by a Bioreactor with Repeated Coupling of Aerobes and Anaerobes Aiming at On-site Reduction of Excess Sludge Water Science and Technology : a Journal of the International Association on Water Pollution Research. 2006 | Pubmed ID: 16841729 A Simplified Method for Assay of Hydrogenase Activities of H2 Evolution and Uptake in Enterobacter Aerogenes Biotechnology Letters. Jul, 2005 | Pubmed ID: 16132849 Rapid Detection of a Gfp-marked Enterobacter Aerogenes Under Anaerobic Conditions by Aerobic Fluorescence Recovery FEMS Microbiology Letters. Aug, 2005 | Pubmed ID: 16006057 Geautomatiseerde microbiële teelt en adaptieve evolutie met behulp van microbieel microdruppelcultuursysteem (MMC) Xingjin Jian*1,2, Xiaojie Guo*1,2,3, Jia Wang4, Zheng Lin Tan1,2,6, Xin-hui Xing1,2,5, Liyan Wang3, Chong Zhang1,2,5 1Department of Chemical Engineering, Institute of Biochemical Engineering, Tsinghua University, 2Key Laboratory of Industrial Biocatalysis, Ministry of Education, Tsinghua University, 3Luoyang TMAXTREE Biotechnology Co., Ltd., 4Biochemical Engineering Research Group, School of Chemical Engineering and Technology, Xi’an Jiaotong University, 5Center for Synthetic & Systems Biology, Tsinghua University, 6School of Life Science and Technology, Tokyo Institute of Technology JoVE 62800 Bioengineering
Geautomatiseerde microbiële teelt en adaptieve evolutie met behulp van microbieel microdruppelcultuursysteem (MMC) Xingjin Jian*1,2, Xiaojie Guo*1,2,3, Jia Wang4, Zheng Lin Tan1,2,6, Xin-hui Xing1,2,5, Liyan Wang3, Chong Zhang1,2,5 1Department of Chemical Engineering, Institute of Biochemical Engineering, Tsinghua University, 2Key Laboratory of Industrial Biocatalysis, Ministry of Education, Tsinghua University, 3Luoyang TMAXTREE Biotechnology Co., Ltd., 4Biochemical Engineering Research Group, School of Chemical Engineering and Technology, Xi’an Jiaotong University, 5Center for Synthetic & Systems Biology, Tsinghua University, 6School of Life Science and Technology, Tokyo Institute of Technology JoVE 62800 Bioengineering