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In JoVE (1)
Other Publications (1)
Articles by Yoon Hyeun Oum in JoVE
Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry
Frederick A. Rubino, Yoon Hyeun Oum, Lakshmi Rajaram, Yanjie Chu, Isaac S. Carrico
Department of Chemistry, Stony Brook University
Adenovirus particles are engineered to contain either the unnatural amino acid analogue azidohomoalanine or the azido sugar O-GlcNAz. The azide group of each is chemoselectively ligated via "click" chemistry reactions as a means of viral surface modification.
Other articles by Yoon Hyeun Oum on PubMed
Bioconjugate Chemistry. Jul, 2012 | Pubmed ID: 22681483
Methods for targeting oncolytic viruses can increase efficacy and accelerate development. Genetic engineering, the predominant method for changing vector tropism, is limited in scope and often represents the bottleneck for vector development. Metabolic incorporation of an unnatural azido sugar, O-GlcNAz, at a specific site on the adenoviral surface allows chemoselective attachment of affibodies for Her2 or EGF receptors. Modification with these high-affinity, high-selectivity proteins is straightforward and readily generalizable, demonstrates minimal impact on virus physiology, and affords significant increases in gene delivery to cancer cells. As a result, this method has significant potential to increase the efficacy of next-generation viral vectors.