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Exploring Cellular Zinc Regulation Through ZnT1 Functionality (Video) | JoVE
Zinc-Specific Staining of Transfected HEK293T Cells (Video) | JoVE
Sample Preparation and Fluorescence Measurement of Stained Transfected HEK293T Cells (Video) | JoVE
由于与蛋白质功能的因果关系较弱且时间分辨率低,锌转运已被证明具有挑战性。该协议描述了一种通过使用Zn 2 +敏感荧光染料以高时间分辨率监测活细胞中Zn 2 +挤出的方法,从而提供Zn2 +外排的直接测量。
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