3D Culture of Organoids from Murine Intestinal Crypts and Single Stem Cell

April 7th, 2023

The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research (Video) | JoVE

02:35 min

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Processing Murine Small Intestine for Crypt Isolation (Video) | JoVE

01:35 min

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Crypt Isolation from Murine Small Intestine (Video) | JoVE

05:06 min

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Organoid Culture from a Single Intestinal Stem Cell (Video) | JoVE

02:05 min

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Yuta Takase¹, Kazuto Fujishima^2,3, Toshio Takahashi¹

¹Suntory Foundation for Life Sciences, Bioorganic Research Institute, ²Institute for Integrated Cell-Material Sciences (KUIAS-iCeMS), Kyoto University, ³Faculty of Medicine, Osaka Medical and Pharmaceutical University

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Murin ince bağırsak kriptlerini ve kültür bağırsak 3D organoidlerini kriptlerden izole etmek için bir protokol tarif ediyoruz. Ek olarak, bir alt epitelyal hücresel nişin yokluğunda tek bir bağırsak kök hücresinden organoidler üretmek için bir yöntem tarif ediyoruz.

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3D Culture of Organoids from Murine Intestinal Crypts and Single Stem Cell

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