High-Throughput Quantification of the Synthesis and Distribution of mtDNA
May 5th, 2023High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution (Video) | JoVE
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Cell Transfection and 5-Bromo-2'-Deoxyuridine (BrdU) Incorporation in HeLa Cells (Video) | JoVE
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5-Bromo-2'-Deoxyuridine (BrdU) Labeling of Mitochondrial DNA in HeLa Cells (Video) | JoVE
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Summary
描述了使用多孔板格式和自动免疫荧光成像来检测和量化mtDNA合成和分布的细胞中线粒体DNA(mtDNA)代谢动力学的程序。这可以进一步用于研究各种抑制剂,细胞应激和基因沉默对mtDNA代谢的影响。