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Intracellular Signaling Events and Hormone Secretion in Human Pseudoislets

November 3rd, 2023
*1, *1, 1, 2, 3, 2, 2, 2, 3, 1,2,4, 2
1Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, 2Division of Diabetes, Endocrinology and Metabolism, Department of Medicine, Vanderbilt University Medical Center, 3Department of Biomedical Engineering, University of Miami, 4VA Tennessee Valley Healthcare System
* These authors contributed equally

Summary
Automatic Translation

Denne protokol beskriver en metode til synkron erhvervelse og medregistrering af intracellulære signalhændelser og udskillelse af insulin og glukagon af primære humane pseudoislets ved hjælp af adenoviral levering af en cyklisk adenosinmonophosphat (cAMP) biosensor, en cAMP-differensdetektor in situ (cADDis) og et mikroperifusionssystem.

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