In Vivo Brain Tumor Modeling via Electroporation-Based Delivery of Plasmid DNA

June 23rd, 2023

Modeling Brain Tumors In Vivo Using Electroporation-Based Delivery of Plasmid DNA Representing Patient Mutation Signatures (Video) | JoVE

02:03 min

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Microinjection of DNA Plasmid Mix into the Mouse Brain Ventricle (Video) | JoVE

00:53 min

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Electroporation-Based DNA Plasmids Delivery to Induce In Vivo Mutations in the Mouse (Video) | JoVE

01:32 min

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Katie B. Grausam*¹, Joshua J. Breunig*^1,2,3,4,5

¹Board of Governor’s Regenerative Medicine Institute, Cedars-Sinai Medical Center, ²Center for Neural Sciences in Medicine, Cedars-Sinai Medical Center, ³Department of Biomedical Sciences, Cedars-Sinai Medical Center, ⁴Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, ⁵Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles

* These authors contributed equally

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Die Verwendung eines immunkompetenten, autochthonen Tumormodells, das von häufigen Patientenmutationen angetrieben wird, für präklinische Tests ist für immuntherapeutische Tests von entscheidender Bedeutung. Dieses Protokoll beschreibt eine Methode zur Generierung von Hirntumor-Mausmodellen unter Verwendung der elektroporationsbasierten Verabreichung von Plasmid-DNA, die häufige Patientenmutationen repräsentieren, und liefert so ein genaues, reproduzierbares und konsistentes Mausmodell.

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In Vivo Brain Tumor Modeling via Electroporation-Based Delivery of Plasmid DNA

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