A Comprehensive Approach to Studying the Promoter Interactome in Scarce Cells

April 21st, 2023

An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations (Video) | JoVE

01:54 min

•

Lysis and Digestion of Scarce Cells (Video) | JoVE

01:16 min

•

Ligation and Decrosslinking of Scarce Cell DNA (Video) | JoVE

01:06 min

•

Sonication and Biotin Pull-Down of Scarce Cells DNA (Video) | JoVE

01:47 min

•

DATP-Tailing, Adapter Ligation, and PCR Amplification of Scarce Cell DNA (Video) | JoVE

03:34 min

•

Library Capture, Biotin Pull-Down, and PCR Amplification Using Scarce Cell DNA Library (Video) | JoVE

02:37 min

•

Llorenç Rovirosa*¹, Laureano Tomás-Daza*^1,2, Blanca Urmeneta¹, Alfonso Valencia^2,3, Biola M. Javierre^1,4

¹Josep Carreras Leukaemia Research Institute, ²Barcelona Supercomputing Center, ³Catalan Institution for Research and Advanced Studies (ICREA), ⁴Institute for Health Science Research Germans Trias i Pujol

* These authors contributed equally

Summary
Automatic Translation

English (Original)
العربية (Arabic)
中文 (Chinese)
dansk (Danish)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
हिंदी (Hindi)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
norsk (Norwegian)
português (Portugese)
русский (Russian)
español (Spanish)
svenska (Swedish)
Türkçe (Turkish)

Genekspression reguleres af interaktioner mellem genpromotorer med distale regulatoriske elementer. Her beskriver vi, hvordan lavt input Capture Hi-C (liCHi-C) gør det muligt at identificere disse interaktioner i sjældne celletyper, som tidligere var umålelige.

Article

A Comprehensive Approach to Studying the Promoter Interactome in Scarce Cells

Get cutting-edge science videos from JoVE sent straight to your inbox every month.