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Ex Vivo Protocol for Culturing Quiescent Muscle Stem Cells with Niche Components (Video) | JoVE
Harvesting and Digesting Mouse Skeletal Muscle for 2D Culture of Niche and Stem Cells (Video) | JoVE
骨骼肌由多种细胞类型组成,包括常驻干细胞,每种细胞都对肌肉稳态和再生有特殊贡献。在这里,描述了肌肉干细胞的 2D 培养和肌肉细胞生态位在 离体 环境中保留了许多生理、 体内和环境特征。
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