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Analyzing Mitochondrial Physiology and Bioenergetics in Fruit Flies

November 10th, 2023

Exploring Mitochondrial Function and Chemical Toxicity Using Drosophila melanogaster (Video) | JoVE

Exploring Mitochondrial Function and Chemical Toxicity Using Drosophila melanogaster (Video) | JoVE

02:34 min

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Calibrating Polarographic Oxygen Sensors for High-Resolution Respirometry Analysis in Drosophila melanogaster (Video) | JoVE

Calibrating Polarographic Oxygen Sensors for High-Resolution Respirometry Analysis in Drosophila melanogaster (Video) | JoVE

01:38 min

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Substrate-Uncoupler-Inhibitor-Titration for Mitochondrial Respiration Analysis in Drosophila melanogaster (Video) | JoVE

Substrate-Uncoupler-Inhibitor-Titration for Mitochondrial Respiration Analysis in Drosophila melanogaster (Video) | JoVE

05:12 min

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Paula Michelotti*^1,2, Tâmie Duarte*^1,2, Cristiane L. Dalla Corte^1,2

¹Laboratory of Experimental Biochemistry and Toxicology, Department of Biochemistry and Molecular Biology, Center of Natural and Exact Sciences, Federal University of Santa Maria, ²Graduate Program in Biological Sciences: Toxicological Biochemistry, Federal University of Santa Maria

* These authors contributed equally

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여기서는 PINK1^B9-null 돌연변이 초파리의 생체 에너지를 분석하기 위한 고분해능 호흡 측정 프로토콜을 제시합니다. 이 분석법은 SUIT(Substrate-Uncoupler-Inhibitor-Titration) 프로토콜을 사용합니다.

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