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Using Induced Pluripotent Stem Cell Suspension for Kidney Organoid Production

September 1st, 2023
*1,2, *3, 4, 2, 1, 1, 1, 1, 5, 1, 1,2
1Department of Nephrology, The Children's Hospital, Zhejiang University School of Medicine, 2Zhejiang University School of Medicine, 3Hubei Normal University, 4National Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University School of Medicine, 5Clinical Laboratory, The Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health
* These authors contributed equally

Summary
Automatic Translation

Questo protocollo presenta un metodo completo ed efficiente per la produzione di organoidi renali da cellule staminali pluripotenti indotte (iPSC) utilizzando condizioni di coltura in sospensione. L'enfasi principale di questo studio risiede nella determinazione della densità cellulare iniziale e della concentrazione dell'agonista WNT, avvantaggiando così i ricercatori interessati alla ricerca sugli organoidi renali.

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