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MyDoBID- A Novel Technique for Identifying Muscle Fiber Types Efficiently

September 22nd, 2023
*1, *1, 1, 1, 1
1Department of Biochemistry and Chemistry, La Trobe Institute for Molecular Science, School of Agriculture, Biomedicine and Environment, La Trobe University
* These authors contributed equally

Summary
Automatic Translation

This protocol demonstrates single-fiber isolation from freeze-dried human skeletal muscle and fiber-type classification according to Myosin heavy chain (MHC) isoform using the dot blotting technique. Identified MHC I and II fiber samples can then be further analyzed for fiber type-specific differences in protein expression using western blotting.

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