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Quantitative Analysis of Mitophagy in Murine Pancreatic β-Cells

September 15th, 2023

Assessment of Mitophagy Flux in Pancreatic Î²-Cells Using Effective and Robust Complementary Approaches (Video) | JoVE

Assessment of Mitophagy Flux in Pancreatic Î²-Cells Using Effective and Robust Complementary Approaches (Video) | JoVE

01:09 min

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Single-Cell Dissociation of Murine Islets for Flow Cytometric Analysis of Î²-Cell Mitophagy (Video) | JoVE

Single-Cell Dissociation of Murine Islets for Flow Cytometric Analysis of Î²-Cell Mitophagy (Video) | JoVE

01:43 min

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Flow Cytometric Analysis of Mitophagy in Murine Pancreatic Î²-Cells Using MtPhagy Dye (Video) | JoVE

Flow Cytometric Analysis of Mitophagy in Murine Pancreatic Î²-Cells Using MtPhagy Dye (Video) | JoVE

02:37 min

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Flow Cytometric Analysis of Mitophagy in Murine Pancreatic Î²-Cells Using the Genetically Encoded mt-Keima Reporter (Video) | JoVE

Flow Cytometric Analysis of Mitophagy in Murine Pancreatic Î²-Cells Using the Genetically Encoded mt-Keima Reporter (Video) | JoVE

01:45 min

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Elena Levi-D’Ancona^1,2, Vaibhav Sidarala¹, Scott A. Soleimanpour^1,3,4

¹Department of Internal Medicine, Division of Metabolism, Endocrinology and Diabetes, University of Michigan, Ann Arbor, ²Graduate Program in Immunology, University of Michigan Medical School, ³Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, ⁴VA Ann Arbor Healthcare System

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该协议概述了对胰腺β细胞中线粒体自噬进行定量分析的两种方法：第一种是细胞渗透性线粒体特异性染料的组合，第二种是遗传编码的线粒体自噬报告基因。这两种技术是互补的，可以根据特定需求进行部署，从而在定量解决线粒体质量控制问题时具有灵活性和精确性。

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