Detection of G1 Phase Single-Stranded DNA Foci via RPA2 Immunofluorescent Staining

December 22nd, 2023

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Qingyue Zhang^1,2, Marc A. Kerzhnerman^1,2, Nelson García-Vázquez^1,2,3, Gergely Rona^1,2,4,5

¹Department of Biochemistry and Molecular Pharmacology, NYU Grossman School of Medicine, ²The Laura and Isaac Perlmutter Cancer Center, NYU Langone Health, ³Department of Cell Biology, NYU Grossman School of Medicine, ⁴Howard Hughes Medical Institute, NYU Grossman School of Medicine, ⁵Institute of Enzymology, Centre of Excellence of the Hungarian Academy of Sciences, HUN-REN Research Centre for Natural Sciences

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يقدم البروتوكول التالي الكشف عن بؤر الحمض النووي التي تقطعت بها السبل في المرحلة G1 من دورة الخلية باستخدام مزامنة دورة الخلية متبوعة بتلطيخ RPA2 المناعي.

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Detection of G1 Phase Single-Stranded DNA Foci via RPA2 Immunofluorescent Staining

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