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High-Resolution 3D Reconstruction of Human Brain with SHORT and LSFM

January 26th, 2024
*1, *1, *1,2, 1, 1,3, 1, 1,4,5, 1,4,6, 1,4,5
1European Laboratory for Non-linear Spectroscopy (LENS), University of Florence, 2Division of Physiology, Department of Experimental and Clinical Medicine, University of Florence, 3Department of Physics, University of Pisa, 4National Research Council - National Institute of Optics (CNR-INO), 5Department of Physics and Astronomy, University of Florence, 6Department of Biology, University of Florence
* These authors contributed equally

Summary
Automatic Translation

Mevcut protokol, (SWITCH - H2O2 - Antijen Reterival - 2,2'-tiyodietanol [TDE]) KISA doku transformasyon tekniğini rutin olarak yüksek verimli bir protokolde ışık tabakası floresan mikroskobu görüntüleme ile birleştirerek onlarca postmortem insan beyni kesitinin hızlı ve eşzamanlı optik temizleme, çok yönlü etiketleme ve 3D hacimsel rekonstrüksiyonu için adım adım bir prosedür sağlar.

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