JoVE Behavior documents observational and experimental techniques that seek to understand human and animal behavior. Work in this field includes analysis of genetic causes and physiological responses to internal or external stimuli as well as the implicated brain regions.
1Brain Research Institute, University of Zurich, 2Department of Biology and Department of Health Sciences and Technology, ETH Zurich, 3Clinical Neurorehabilitation, Department of Neurology, University of Zurich & University Hospital Zurich
A paradigm is presented to analyze the acquisition of a high-precision skilled forelimb reaching task in rats.
Published June 22, 2015. Keywords: Behavior, Motor Learning, Rat, Skilled Reaching Task, Single Pellet Grasping, Procedural Learning
1Department of Neuroscience, Oberlin College
This protocol describes how to temporarily and remotely silence neuronal activity in discrete brain regions while rats are engaged in learning and memory tasks. The approach combines pharmacogenetics (Designer-Receptors-Exclusively-Activated-by-Designer-Drugs) with a behavioral paradigm (sensory preconditioning) that is designed to distinguish between different components of learning.…
Published June 22, 2015. Keywords: Behavior, Pharmacogenetics, behavior, neuroscience, temporary neural inactivation, sensory preconditioning, rodent, cortex, adeno-associated virus, DREADDs, learning, stereotaxic surgery
1Department of Psychology, Behavioral Neuroscience, West Virginia University, 2Department of Physiology and Pharmacology, West Virginia University, 3Department of Neurology, N. Bud Grossman Center for Memory Research and Care, University of Minnesota, 4Department of Neuroscience, N. Bud Grossman Center for Memory Research and Care, University of Minnesota, 5GRECC, VA Medical Center, 6Center for Neuroscience, Center for Basic and Translational Stroke Research, West Virginia University
This manuscript describes a Morris water maze (MWM) protocol tailored for use with a commonly used mouse model of Alzheimer's disease. The MWM is widely used in transgenic mouse models. Implementation of a procedure sensitive to the background strain of the mouse model is essential for detecting group differences.
Published June 22, 2015. Keywords: Behavior, Spatial learning, spatial reference memory, Morris water maze, Alzheimer’s disease, behavior, tau, hippocampal-dependent learning, rTg4510, Tg2576, strain background, transgenic mouse models
1Unit of Anatomy, Department of Medicine, University of Fribourg
We present a simple and unbiased olfactory test in mice. With this protocol olfactory discrimination, preference, avoidance and sensitivity to a novel odor as compared to water can be assessed in single behavioral sessions. This method is indicated for a single experimenter and analysis is based on computer-assisted video processing.
Published June 15, 2015. Keywords: Behavior, mice, olfactory perception, olfactory sensitivity, peanut butter, urine, 2-methylbutyric acid, computer-assisted video processing
1Department of Psychiatry, Yale School of Medicine, 2Department of Electronics and Bioinformatics, Meiji University, 3Department of Histology and Neurobiology, Dokkyo Medical University School of Medicine, 4ADAM Center, Department of Physical Therapy, Movement and Rehabilitation Sciences, Northeastern University, 5Department of Neurobiology, Yale School of Medicine
We present a method to compare functional brain activity recorded during a naturalistic task using fNIRS with activity recorded during fMRI.
Published June 15, 2015. Keywords: Behavior, fNIRS (functional near-infrared spectroscopy), fMRI (functional magnetic resonance imaging), motor learning, video game, multisensory integration, temporal gyrus, frontopolar, prefrontal cortex
1Department of Molecular Virology, Immunology, and Medical Genetics, and Comprehensive Cancer Center, The Ohio State University
Environmental enrichment for mice requires a complex and challenging setup, as well as comprehensive husbandry and handling techniques to assure robust metabolic and anti-cancer effects in the mice. This protocol provides detailed procedures to reproduce the above mentioned effects in mice.
Published June 8, 2015. Keywords: Behavior, Mouse, Environmental Enrichment, Animal Husbandry, Metabolism, Cancer, Hypothalamic-sympathoneural-adipocyte (HSA) axis.
1Division of Biokinesiology & Physical Therapy at the Herman Ostrow School of Dentistry, University of Southern California, 2Department of Kinesiology, Temple University, 3Department of Physical Therapy, Niigata University of Health and Welfare
A method is described in which 3-4 month old infants learn a task by discovery and their leg movements are captured to quantify the learning process.
Published June 1, 2015. Keywords: Behavior, infant, discovery-learning, motor learning, motor control, kinematics, kinetics
1Speech-Language Pathology Program, Laurentian University, 2Psychology Department, Laurentian University
Children who have complex communication needs can benefit from the use of a speech-generating device (SGD). Cognitive skills were identified as having an impact on the ability to navigate between levels of SGDs. This protocol describes the steps needed for Speech-language Pathologists to assess cognitive skills and navigational abilities.
Published June 1, 2015. Keywords: Behavior, Augmentative and alternative communication, navigation, cognition, assessment, speech-language pathology, children
1Department of Psychiatry and Behavioural Neurosciences, McMaster University, 2McMaster Integrative Neuroscience Discovery and Study, McMaster University, 3Department of Psychiatry, University of Calgary, 4Department of Psychology, Neuroscience & Behaviour, McMaster University
We aim to identify the neural correlates underlying sustained and transient thought suppression, and thought re-emergence in controls, at-risk and depressed individuals. Activation was greatest for controls compared to the at-risk and the depressed group in the dorsolateral prefrontal cortex during thought suppression and anterior cingulate cortex during thought re-emergence.…
Published May 19, 2015. Keywords: Behavior, Major Depressive Disorder, Risk, Thought Suppression, fMRI, Women, Rumination, Thought Intrusion
1Department of Biology, The Pennsylvania State University
Stereotactic injection of lentiviruses expressing cDNAs or shRNAs can modulate gene expression in specific brain areas of mice. Here, we present a protocol to combine stereotactic injections with behavioral tasks, such as the Open Field Test (OFT) and the Forced Swim Test (FST).
Published May 10, 2015. Keywords: Behavior, Stereotactic Injection, Open Field Test, Forced Swimming test, Anxiety, Behavior, Neuroscience