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General Laboratory Techniques

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Basic Methods in Cellular and Molecular Biology

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JoVE Behavior
JoVE Behavior documents observational and experimental techniques that seek to understand human and animal behavior. Work in this field includes analysis of genetic causes and physiological responses to internal or external stimuli as well as the implicated brain regions.
 JoVE Behavior

Acquisition of a High-precision Skilled Forelimb Reaching Task in Rats

1Brain Research Institute, University of Zurich, 2Department of Biology and Department of Health Sciences and Technology, ETH Zurich, 3Clinical Neurorehabilitation, Department of Neurology, University of Zurich & University Hospital Zurich


JoVE 53010

A paradigm is presented to analyze the acquisition of a high-precision skilled forelimb reaching task in rats.

 JoVE Behavior

A Method for Remotely Silencing Neural Activity in Rodents During Discrete Phases of Learning

1Department of Neuroscience, Oberlin College


JoVE 52859

This protocol describes how to temporarily and remotely silence neuronal activity in discrete brain regions while rats are engaged in learning and memory tasks. The approach combines pharmacogenetics (Designer-Receptors-Exclusively-Activated-by-Designer-Drugs) with a behavioral paradigm (sensory preconditioning) that is designed to distinguish between different components of learning.

 JoVE Behavior

Morris Water Maze Test: Optimization for Mouse Strain and Testing Environment

1Department of Psychology, Behavioral Neuroscience, West Virginia University, 2Department of Physiology and Pharmacology, West Virginia University, 3Department of Neurology, N. Bud Grossman Center for Memory Research and Care, University of Minnesota, 4Department of Neuroscience, N. Bud Grossman Center for Memory Research and Care, University of Minnesota, 5GRECC, VA Medical Center, 6Center for Neuroscience, Center for Basic and Translational Stroke Research, West Virginia University


JoVE 52706

This manuscript describes a Morris water maze (MWM) protocol tailored for use with a commonly used mouse model of Alzheimer's disease. The MWM is widely used in transgenic mouse models. Implementation of a procedure sensitive to the background strain of the mouse model is essential for detecting group differences.

 JoVE Behavior

Simple and Computer-assisted Olfactory Testing for Mice

1Unit of Anatomy, Department of Medicine, University of Fribourg


JoVE 52944

We present a simple and unbiased olfactory test in mice. With this protocol olfactory discrimination, preference, avoidance and sensitivity to a novel odor as compared to water can be assessed in single behavioral sessions. This method is indicated for a single experimenter and analysis is based on computer-assisted video processing.

 JoVE Behavior

fMRI Validation of fNIRS Measurements During a Naturalistic Task

1Department of Psychiatry, Yale School of Medicine, 2Department of Electronics and Bioinformatics, Meiji University, 3Department of Histology and Neurobiology, Dokkyo Medical University School of Medicine, 4ADAM Center, Department of Physical Therapy, Movement and Rehabilitation Sciences, Northeastern University, 5Department of Neurobiology, Yale School of Medicine


JoVE 52116

We present a method to compare functional brain activity recorded during a naturalistic task using fNIRS with activity recorded during fMRI.

 JoVE Behavior

A Protocol for Housing Mice in an Enriched Environment

1Department of Molecular Virology, Immunology, and Medical Genetics, and Comprehensive Cancer Center, The Ohio State University


JoVE 52874

Environmental enrichment for mice requires a complex and challenging setup, as well as comprehensive husbandry and handling techniques to assure robust metabolic and anti-cancer effects in the mice. This protocol provides detailed procedures to reproduce the above mentioned effects in mice.

 JoVE Behavior

Quantifying Learning in Young Infants: Tracking Leg Actions During a Discovery-learning Task

1Division of Biokinesiology & Physical Therapy at the Herman Ostrow School of Dentistry, University of Southern California, 2Department of Kinesiology, Temple University, 3Department of Physical Therapy, Niigata University of Health and Welfare


JoVE 52841

A method is described in which 3-4 month old infants learn a task by discovery and their leg movements are captured to quantify the learning process.

 JoVE Behavior

Practical Methodology of Cognitive Tasks Within a Navigational Assessment

1Speech-Language Pathology Program, Laurentian University, 2Psychology Department, Laurentian University


JoVE 52286

Children who have complex communication needs can benefit from the use of a speech-generating device (SGD). Cognitive skills were identified as having an impact on the ability to navigate between levels of SGDs. This protocol describes the steps needed for Speech-language Pathologists to assess cognitive skills and navigational abilities.

 JoVE Behavior

Design and Implementation of an fMRI Study Examining Thought Suppression in Young Women with, and At-risk, for Depression

1Department of Psychiatry and Behavioural Neurosciences, McMaster University, 2McMaster Integrative Neuroscience Discovery and Study, McMaster University, 3Department of Psychiatry, University of Calgary, 4Department of Psychology, Neuroscience & Behaviour, McMaster University


JoVE 52061

We aim to identify the neural correlates underlying sustained and transient thought suppression, and thought re-emergence in controls, at-risk and depressed individuals. Activation was greatest for controls compared to the at-risk and the depressed group in the dorsolateral prefrontal cortex during thought suppression and anterior cingulate cortex during thought re-emergence.

 JoVE Behavior

Applying Stereotactic Injection Technique to Study Genetic Effects on Animal Behaviors

1Department of Biology, The Pennsylvania State University


JoVE 52653

Stereotactic injection of lentiviruses expressing cDNAs or shRNAs can modulate gene expression in specific brain areas of mice. Here, we present a protocol to combine stereotactic injections with behavioral tasks, such as the Open Field Test (OFT) and the Forced Swim Test (FST).

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