JoVE Bioengineering merges both physical and life sciences to understand and predict biological processes. Applying physical science tools to life science questions allow for the discovery of better technologies to measure, diagnose, and clinically treat disease.
1MatTek Corporation, 2MatTek In Vitro Life Science Laboratories
We have developed an eye irritation test which utilizes a three dimensional reconstructed human cornea-like epithelial (RhCE) tissue model. The test is able to discriminate between ocular irritant and corrosive materials (GHS Categories 1 and 2 combined) and those that do not require labeling (GHS No Category).
Published August 23, 2015. Keywords: Bioengineering, alternative methods, cornea, EpiOcular, EURL ECVAM, eye irritation, in vitro, MTT viability assay, ocular irritation, REACH, reconstructed ocular tissue model, RhCE
1Department of Biomedical Engineering, National University of Singapore, 2Mechanobiology Institute, Singapore, National University of Singapore, 3Department of Surgery, National University of Singapore
Scaffolds for tissue engineering need to recapitulate the complex biochemical and biophysical microenvironment of the cellular niche. Here, we show the use of interfacial polyelectrolyte complexation fibers as a platform to create composite, multi-component polymeric scaffolds with sustained biochemical release.
Published August 19, 2015. Keywords: Bioengineering, composite scaffold, polymer, hydrogel, biochemicals, encapsulation, temporal, spatial, sustained release, topography
1Department of Physiology, University of Tennessee Health Science Center, 2Department of Biomedical Engineering and Imaging, University of Tennessee Health Science Center, 3Department of Biomedical Engineering, University of Memphis, 4Department of Engineering Technology, University of Memphis
A novel imaging protocol was developed using a custom motor-driven mechanical actuator to allow the measurement of real time responses to mechanical strain in live cells. Relevant to mechanobiology, the system can apply strains up to 20% while allowing near real-time imaging with confocal or atomic force microscopy.
Published August 19, 2015. Keywords: Bioengineering, Mechanotransduction, bioreactor, mechanobiology, overdistention, pulmonary epithelium, acute lung injury
1Comprehensive Transplant Center, Feinberg School of Medicine, Northwestern University, 2Department of Surgery, Feinberg School of Medicine, Northwestern University, 3Department of Biomedical Engineering, Northwestern University, 4Simpson Querrey Institute for BioNanotechnology in Medicine, Northwestern University, 5Department of Internal Medicine, University of New Mexico HSC, 6Department of Pathology, University of New Mexico HSC, 7Department of Chemical and Biological Engineering, Northwestern University, 8Chemistry of Life Processes Institute, Northwestern University, 9Department of Surgery, Jesse Brown VA Medical Center
This protocol describes decellularization of Sprague Dawley rat kidneys by antegrade perfusion of detergents through the vasculature, producing acellular renal extracellular matrices that serve as templates for repopulation with human renal epithelial cells. Recellularization and use of the resazurin perfusion assay to monitor growth is performed within specially-designed perfusion bioreactors.…
Published August 10, 2015. Keywords: Bioengineering, Bioreactor, cell seeding, decellularization, extracellular matrix, epithelial, kidney, perfusion, recellularization, renal, resazurin, scaffold, tissue engineering
1General, Visceral, and Transplantation Surgery, Charité – Universitätsmedizin Berlin
The presented techniques for liver harvesting, cannulation and perfusion using our proprietary device enable sophisticated perfusion set-ups to improve decellularization and recellularization experiments in rat livers.
Published August 10, 2015. Keywords: Bioengineering, Rat Liver Decellularization, Rat Liver Perfusion Device, Oscillating Pressure Conditions, Rat Liver Engineering, Arterial Rat Liver Perfusion, Portal Venous Rat Liver Perfusion
1Center for Biomaterials and Tissue Engineering, Universitat Politècnica de València, 2Division of Biomedical Engineering, School of Engineering, University of Glasgow, 3Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN)
The following protocol describes the procedure to assemble sandwich-like cultures to be used as an intermediate stage between bi-dimensional (2D) and three-dimensional (3D) cellular environments. The engineered systems can have applications in microscopy, biomechanics, biochemistry and cell biology assays.
Published August 4, 2015. Keywords: Bioengineering, Sandwich culture, 3D culture, cell culture, physiological, cellular environment, bioengineering
1Woodruff School of Mechanical Engineering, Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, 2Coulter Department of Biomedical Engineering, Georgia Institute of Technology, 3Charles Perkins Centre, The University of Sydney, 4Institute of Biophysics, Laboratory of RNA Biology, Chinese Academy of Sciences, 5University of Chinese Academy of Sciences, 6School of Medicine and Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University
We describe a technique for concurrently measuring force-regulated single receptor-ligand binding kinetics and real-time imaging of calcium signaling in a single T lymphocyte.
Published August 4, 2015. Keywords: Bioengineering, single cell, single molecule, receptor-ligand binding, kinetics, fluorescence and force spectroscopy, adhesion, mechano-transduction, calcium
1Division of Drug Delivery and Tissue Engineering, University of Nottingham, 2Laboratory of Biophysics and Surface Analysis, School of Pharmacy, University of Nottingham, 3Division of Immunology and Allergy, School of Molecular Medical Sciences, University of Nottingham, 4Division of Respiratory Medicine, School of Clinical Sciences, University of Nottingham, 5NIHR Respiratory Biomedical Research Unit, University of Leicester, 6School of Sport, Exercise, and Health Sciences, Loughborough University
Advancements in biomaterial technologies enable the development of three-dimensional multi-cell-type constructs. We have developed electrospinning protocols to produce three individual scaffolds to culture the main structural cells of the airway to provide a 3D in vitro model of the airway bronchiole wall.
Published July 31, 2015. Keywords: Bioengineering, Electrospinning, 3D Cell Culture, Bioreactor, Airway, Tissue Engineering, In Vitro Model
1Graduate School of Medicine, Laboratory of Clinical Biotechnology, The University of Tokyo, 2Graduate School of Engineering, Department of Materials Engineering, The University of Tokyo, 3Graduate School of Engineering, Department of Bioengineering, The University of Tokyo
This protocol describes a cell transplantation system using genetically modified, injectable spheroids. Cell spheroids are cultured on micropatterned culture plates and recovered after gene introduction using polyplex nanomicelles. This system facilitates prolonged transgene expression from the transplanted cells in host animals while maintaining the innate function of the cells.…
Published July 31, 2015. Keywords: Bioengineering, Spheroid, Cell transplantation, Gene transfection, Non-viral carrier, Micropatterned plate, Genetic modification
1Interfaculty Institute for Biochemistry, 2Max Planck Institute for Intelligent Systems, 3German Cancer Research Center
We describe a protocol for preparation of supported lipid bilayers and its characterization using atomic force microscopy and force spectroscopy.
Published July 22, 2015. Keywords: Bioengineering, supported lipid bilayers, atomic force microscopy, force spectroscopy, lipid rafts, liquid ordered phase, breakthrough force, lipid membranes