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JoVE Biology
JoVE Biology welcomes all general biology research methodologies. Content in this section canvases all fields of cell, molecular, and organismal biology, ranging from new applications of standard techniques to novel approaches aimed at understanding the functions of life and living organisms. This diverse section includes, but is not limited to, techniques in physical biology, cellular biochemistry, genetics, physiology, systems biology and a combination of eukaryotic and prokaryotic model systems.
 JoVE Biology

Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis

1Department of Anesthesiology and Perioperative Care, University of California Irvine, 2Department of Biomedical and Molecular Sciences, Queen's University, 3Department of Pharmacology, University of California Irvine


JoVE 52824

Receptor trafficking modulates signaling and cell responsiveness to ligands and is, itself, responsive to cell conditions, including ligand-induced signaling. Here, we describe a powerful and flexible technique for quantitatively assessing drug-induced receptor trafficking using immunolabeling and colocalizational analysis.

 JoVE Biology

Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli

1Department of Physiology, Medical College of Wisconsin


JoVE 52850

Changes in the intracellular calcium levels in the podocytes are one of the most important means to control the filtration function of glomeruli. Here we explain a high-throughput approach that allows detection of real-time calcium handling and single ion channels activity in the podocytes of the freshly isolated glomeruli.

 JoVE Biology

Transfecting RAW264.7 Cells with a Luciferase Reporter Gene

1Immunity and Infection Research Centre, Vancouver Costal Health Research Institute, 2Department of Surgery, University of British Columbia, 3Department of Biochemistry and Molecular Biology, University of British Columbia


JoVE 52807

Transfection into the macrophage cell line, RAW264.7, is difficult due to the cell’s natural response against foreign materials. We described here a gentle yet robust procedure for transfecting luciferase reporter genes into RAW264.7 cells.

 JoVE Biology

Using Mouse Mammary Tumor Cells to Teach Core Biology Concepts: A Simple Lab Module

1Department of Natural Sciences, Marymount Manhattan College


JoVE 52528

A feasible laboratory module for biology undergraduates that explores advanced cellular and molecular concepts using animal cell culture is described. Students grow, characterize and manipulate a breast cancer cell model by exposure to chemotherapy agents. Cell viability is assayed through cell counting using both a standard and novel method.

 JoVE Biology

A High Yield and Cost-efficient Expression System of Human Granzymes in Mammalian Cells

1Cellular and Molecular Medicine Program, Boston Children’s Hospital and Harvard Medical School, 2Department of Medicine, University of Fribourg, 3Centre Médical Universitaire, University of Geneva


JoVE 52911

We describe here a cost-efficient granzyme expression system using HEK293T cells that produces high yields of pure, fully glycosylated and enzymatically active protease.

 JoVE Biology

HeLa Based Cell Free Expression Systems for Expression of Plasmodium Rhoptry Proteins

1Department of Biological, Geological, and Environmental Sciences, Cleveland State University


JoVE 52772

Expression of malarial proteins in cell based systems remains challenging. We demonstrate two step and one step IVT (in vitro translation) cell free expression systems for expressing malarial recombinant rhoptry proteins from HeLa cells. We use a Ni-resin affinity based purification system to purify the rhoptry proteins.

 JoVE Biology

Two-photon Imaging of Intracellular Ca2+ Handling and Nitric Oxide Production in Endothelial and Smooth Muscle Cells of an Isolated Rat Aorta

1Departments of Physiology, Medical College of Wisconsin, 2Human and Molecular Genetics Center, Medical College of Wisconsin, 3Cardiovascular Center, Medical College of Wisconsin, 4Blood Research Institute of Wisconsin


JoVE 52734

Vascular cell functiondepends on activity of intracellular messengers. Described here is an ex vivo two photon imaging method that allows the measurement of intracellular calcium and nitric oxide levels in response to physiological and pharmacological stimuli in individual endothelial and smooth muscle cells of an isolated aorta.

 JoVE Biology

SpOT the Correct Tissue Every Time in Multi-tissue Blocks

1Center for Advanced Preclinical Research (CAPR), Frederick National Laboratory for Cancer Research, 2Leidos Biomedical Research, Inc., 3Department of Oncology, Georgetown University


JoVE 52868

The purpose of the Specimen Orientation Tag (SpOT) is to function as an orientation tool to aid in individual tissue identification in multi-tissue paraffin blocks. These protocols demonstrate how it is constructed easily from common, low-cost histology materials and serves as a reliable visual marker in paraffin blocks and sections.

 JoVE Biology

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

1Department of Biochemistry and Molecular Pharmacology, Institute for Systems Genetics, 2Roche Life Science, USA


JoVE 52941

Designer chromosomes of the Synthetic Yeast Genome project, Sc2.0, can be distinguished from their native counterparts using a PCR-based genotyping assay called PCRTagging, which has a presence/absence endpoint. Here we describe a high-throughput real time PCR detection method for PCRTag genotyping.

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