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Basic Methods in Cellular and Molecular Biology

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JoVE Biology
 JoVE Biology

High-throughput Titration of Luciferase-expressing Recombinant Viruses

1Center for Innovative Cancer Research, Ottawa Hospital Research Institute, 2Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, 3Faculty of Medicine, University of Ottawa


JoVE 51890

This article presents a high-throughput luciferase expression-based method of titrating various RNA and DNA viruses using automated and manual liquid handlers.

 JoVE Biology

Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria

1Department of Biochemistry, Université de Montréal, CRCHUM, 2Department of Neurosciences, Université de Montréal, CRCHUM


JoVE 51887

Described here is a method to detect and quantify mitochondrial outer membrane proteins by immunolabeling of mitochondria isolated from rodent tissue and analysis by flow cytometry. This method can be extended to assess functional aspects of mitochondrial subpopulations.

 JoVE Biology

Stimulation of Cytoplasmic DNA Sensing Pathways In Vitro and In Vivo

1Department of Pathology, University of Cambridge


JoVE 51593

The aim of the protocol is to use optimal methods for stimulating the cytoplasmic DNA sensing pathways in cells and in vivo. This is achieved by improving the generation of long, double-stranded DNA during blunt-end ligation. Cells or mice are then transfected using a lipid transfection reagent.

 JoVE Biology

Visualization of ATP Synthase Dimers in Mitochondria by Electron Cryo-tomography

1Department of Structural Biology, Max Planck Institute of Biophysics


JoVE 51228

We present a protocol of how to collect and process electron cryo-tomograms of whole mitochondria. The technique provides detailed insights into the structure, function, and organization of large membrane protein complexes in native biological membranes.

 JoVE Biology

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry

1Department of Biosciences, University of Exeter


JoVE 51809

Differential scanning fluorimetry is a widely used method for screening libraries of small molecules for interactions with proteins. Here, we present a straightforward method to extend these analyses to provide an estimate of the dissociation constant between a small molecule and its protein partner.

 JoVE Biology

Generation of Transgenic Hydra by Embryo Microinjection

1Yale Stem Cell Center and Department of Cell Biology, Yale University School of Medicine, 2Department of Biological Chemistry and the Developmental Biology Center, University of California, Irvine


JoVE 51888

Stably transgenic Hydra are made by microinjection of plasmid DNA into embryos followed by random genomic integration and asexual propagation to establish a uniform line. Transgenic Hydra are used to track cell movements, overexpress genes, study promoter function, or knock down gene expression using RNAi.

 JoVE Biology

Ex vivo Culture of Drosophila Pupal Testis and Single Male Germ-line Cysts: Dissection, Imaging, and Pharmacological Treatment

1Fachbereich Biologie, Entwicklungsbiologie, Philipps-Universität Marburg, 2Institut für Molekularbiologie und Tumorforschung, Philipps-Universität Marburg


JoVE 51868

This protocol describes the dissection and cultivation of intact testes and germ-line cysts from Drosophila melanogaster pupae. This method allows microscopic observation of spermatogenesis ex vivo. Furthermore, we describe a pharmacological assay of the effect of inhibitors on specific stages of germ-cell development in pupal testes.

 JoVE Biology

Fundamental Technical Elements of Freeze-fracture/Freeze-etch in Biological Electron Microscopy

1Department of Pediatrics, Center for Environmental Medicine, Asthma, and Lung Biology, The University of North Carolina at Chapel Hill


JoVE 51694

Basic techniques and refinements of freeze-fracture processing of biological specimens and nanomaterials for examination by transmission electron microscopy are described. This technique is a preferred method for revealing ultrastructural features and specializations of biological membranes and for obtaining ultrastructural level dimensional and spatial data in materials sciences and nanotechnology products.

 JoVE Biology

Using plusTipTracker Software to Measure Microtubule Dynamics in Xenopus laevis Growth Cones

1Department of Biology, Boston College


JoVE 52138

The MATLAB-based, open source software package, plusTipTracker, can be used to analyze image series of fluorescently-labeled +TIPs to quantify microtubule dynamics.

 JoVE Biology

Isolation and Physiological Analysis of Mouse Cardiomyocytes

1Department of Medicine, Vanderbilt University, 2Department of Cell and Developmental Biology, Vanderbilt University


JoVE 51109

Individual cardiomyocytes from wild type and mutant mice can be isolated from the heart in order to study their contractility and calcium transients. This allows characterization of the contribution of cellular dysfunction to heart dysfunction from any cause.

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