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JoVE Biology
 JoVE Biology

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy (f3D-SIM)

1The ithree Institute, University of Technology, Sydney


JoVE 51469

Spatiotemporal information about dynamic proteins inside live cells is crucial for understanding biology. A type of super-resolution microscopy called fast 3D-structured illumination microscopy (f3D-SIM) reveals unique information about the cytokinetic Z ring in bacteria: both its bead-like appearance and the rapid dynamics of FtsZ within the ring.

 JoVE Biology

Isolating Primary Melanocyte-like Cells from the Mouse Heart

1Penn Cardiovascular Institute, Perelman School of Medicine, University of Pennsylvania


JoVE 4357

In this protocol, we identified a novel population of melanocyte-like cells (also known as cardiac melanocytes) in the hearts of mice and humans that contribute to atrial arrhythmia triggers in mice. 

 JoVE Biology

Fluorescence Imaging with One-nanometer Accuracy (FIONA)

1Department of Physics, University of Illinois at Urbana-Champaign, 2Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, 3Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign


JoVE 51774

Single fluorophores can be localized with nanometer precision using FIONA. Here a summary of the FIONA technique is reported, and how to carry out FIONA experiments is described.

 JoVE Biology

High Efficiency Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes and Characterization by Flow Cytometry

1Department of Biochemistry, Medical College of Wisconsin, 2Stanford Cardiovascular Institute, Stanford University School of Medicine, 3Department of Anesthesiology, Medical College of Wisconsin, 4Stem Cell and Regenerative Medicine Consortium, LKS Faculty of Medicine, Hong Kong University, 5Division of Cardiology, Johns Hopkins University School of Medicine, 6Cardiovascular Research Center, Biotechnology and Bioengineering Center, Medical College of Wisconsin


JoVE 52010

The article describes the detailed methodology to efficiently differentiate human pluripotent stem cells into cardiomyocytes by selectively modulating the Wnt pathway, followed by flow cytometry analysis of reference markers to assess homogeneity and identity of the population.

 JoVE Biology

Protein Isolation from the Developing Embryonic Mouse Heart Valve Region

1McAllister Heart Institute, University of North Carolina at Chapel Hill, 2New York-Presbyterian Hospital/Weill-Cornell Medical Center


JoVE 51911

The analysis of protein expression in young embryonic mouse valves has been hampered by the limited tissue available. This manuscript provides a protocol for preparing protein from developing embryonic mouse valve regions for western blot analysis.

 JoVE Biology

Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster

1Department of Biology, Indiana University


JoVE 51792

The adult structures of Drosophila are derived from sac-like structures called imaginal discs. Analysis of these discs provides insight into many developmental processes including tissue determination, compartment boundary establishment, cell proliferation, cell fate specification, and planar cell polarity. This protocol is used to prepare imaginal discs for light/fluorescent microscopy.

 JoVE Biology

High-throughput Titration of Luciferase-expressing Recombinant Viruses

1Center for Innovative Cancer Research, Ottawa Hospital Research Institute, 2Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, 3Faculty of Medicine, University of Ottawa


JoVE 51890

This article presents a high-throughput luciferase expression-based method of titrating various RNA and DNA viruses using automated and manual liquid handlers.

 JoVE Biology

Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria

1Department of Biochemistry, Université de Montréal, CRCHUM, 2Department of Neurosciences, Université de Montréal, CRCHUM


JoVE 51887

Described here is a method to detect and quantify mitochondrial outer membrane proteins by immunolabeling of mitochondria isolated from rodent tissue and analysis by flow cytometry. This method can be extended to assess functional aspects of mitochondrial subpopulations.

 JoVE Biology

Stimulation of Cytoplasmic DNA Sensing Pathways In Vitro and In Vivo

1Department of Pathology, University of Cambridge


JoVE 51593

The aim of the protocol is to use optimal methods for stimulating the cytoplasmic DNA sensing pathways in cells and in vivo. This is achieved by improving the generation of long, double-stranded DNA during blunt-end ligation. Cells or mice are then transfected using a lipid transfection reagent.

 JoVE Biology

Visualization of ATP Synthase Dimers in Mitochondria by Electron Cryo-tomography

1Department of Structural Biology, Max Planck Institute of Biophysics


JoVE 51228

We present a protocol of how to collect and process electron cryo-tomograms of whole mitochondria. The technique provides detailed insights into the structure, function, and organization of large membrane protein complexes in native biological membranes.

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