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  JoVE Developmental Biology

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JoVE Biology
JoVE Biology welcomes all general biology research methodologies. Content in this section canvases all fields of cell, molecular, and organismal biology, ranging from new applications of standard techniques to novel approaches aimed at understanding the functions of life and living organisms. This diverse section includes, but is not limited to, techniques in physical biology, cellular biochemistry, genetics, physiology, systems biology and a combination of eukaryotic and prokaryotic model systems.
 JoVE Biology

Physiology Lab Demonstration: Glomerular Filtration Rate in a Rat

1Tactical Combat Casualty Care Research, U.S. Army Institute of Surgical Research, 2Department of Pharmacology and Toxicology, Michigan State University, 3Southwest National Primate Research Center, Texas Biomedical Research Institute


JoVE 52425

The purpose of this protocol is to demonstrate the principles and techniques for measuring and calculating glomerular filtration rate, urine flow rate, and excretion of sodium and potassium in a rat. This demonstration can be used to provide students with an overall conceptual understanding of how to measure renal function.

 JoVE Biology

Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis

1Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, 2Proteomics Platform, Broad Institute


JoVE 53057

This protocol describes a procedure for enriching ECM proteins from tissues or tumors and deglycosylating and digesting the ECM-enriched preparations into peptides to analyze their protein composition by mass spectrometry.

 JoVE Biology

Correlative Confocal and 3D Electron Microscopy of a Specific Sensory Cell

1Department of Medicine, Duke University Medical Center, 2Department of Chemistry, University of North Carolina - Chapel Hill, 3Renovo Neural Incorporated


JoVE 52918

Here, we introduce a method, cocem3D, to unveil the ultrastructure of a specific cell in its native tissue by bridging confocal and serial block-face scanning electron microscopy.

 JoVE Biology

Cryosectioning Method for Microdissection of Murine Colonic Mucosa

1Epithelial Pathobiology and Mucosal Inflammation Research Unit, Department of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia


JoVE 53112

Here we describe a simple method for the isolation of spatially distinct murine colonic epithelial surface cells from the underlying crypt-base cells.

 JoVE Biology

Measurement of Heme Synthesis Levels in Mammalian Cells

1Department of Molecular and Cell Biology, Center for Systems Biology, University of Texas at Dallas


JoVE 51579

Altered intracellular heme levels are associated with common diseases such as cancer. Thus, there is a need to measure heme biosynthesis levels in diverse cells. The goal of this protocol is to provide a fast and sensitive method to measure and compare the levels of heme synthesis in different cells.

 JoVE Biology

Bone Conditioned Medium: Preparation and Bioassay

1Department of Oral Surgery and Stomatology, School of Dental Medicine, University of Bern, 2Laboratory of Oral Cell Biology, School of Dental Medicine, University of Bern, 3Department of Oral and Maxillofacial Surgery, School of Dental Medicine, Universitat Internacional de Catalunya, 4Robert K. Schenk Laboratory of Oral Histology, School of Dental Medicine, University of Bern, 5Department of Cranio Maxillofacial Surgery, Inselspital, University of Bern, 6Department of Implant Dentistry, School of Dentistry, Universidade Federal de Santa Catarina


JoVE 52707

We describe here how to prepare bone-conditioned medium (BCM) and test its activity in vitro.

 JoVE Biology

Measuring DNA Damage and Repair in Mouse Splenocytes After Chronic In Vivo Exposure to Very Low Doses of Beta- and Gamma-Radiation

1Radiological Protection Research and Instrumentation, Canadian Nuclear Laboratories


JoVE 52912

A protocol to evaluate changes in DNA damage levels and DNA repair capacity that may be induced by chronic in vivo low dose irradiation in mouse spleen lymphocytes, by measuring phosphorylated histone H2AX, a marker of DNA double-strand breaks, using flow cytometry is presented.

 JoVE Biology

Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis

1Department of Anesthesiology and Perioperative Care, University of California Irvine, 2Department of Biomedical and Molecular Sciences, Queen's University, 3Department of Pharmacology, University of California Irvine


JoVE 52824

Receptor trafficking modulates signaling and cell responsiveness to ligands and is, itself, responsive to cell conditions, including ligand-induced signaling. Here, we describe a powerful and flexible technique for quantitatively assessing drug-induced receptor trafficking using immunolabeling and colocalizational analysis.

 JoVE Biology

Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli

1Department of Physiology, Medical College of Wisconsin


JoVE 52850

Changes in the intracellular calcium levels in the podocytes are one of the most important means to control the filtration function of glomeruli. Here we explain a high-throughput approach that allows detection of real-time calcium handling and single ion channels activity in the podocytes of the freshly isolated glomeruli.

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