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  JoVE Biology

  
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  JoVE Bioengineering

  
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  JoVE Developmental Biology

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JoVE Biology
JoVE Biology welcomes all general biology research methodologies. Content in this section canvases all fields of cell, molecular, and organismal biology, ranging from new applications of standard techniques to novel approaches aimed at understanding the functions of life and living organisms. This diverse section includes, but is not limited to, techniques in physical biology, cellular biochemistry, genetics, physiology, systems biology and a combination of eukaryotic and prokaryotic model systems.
 JoVE Biology

Determination of Fatty Acid Oxidation and Lipogenesis in Mouse Primary Hepatocytes

1Division of Cardiovascular Medicine, Department of Medicine, University of Massachusetts Medical School


JoVE 52982

De novo lipogenesis and β-fatty acid oxidation constitute key metabolic pathways in hepatocyte, pathways that are perturbed in several metabolic disorders, including fatty liver disease. Here we demonstrate isolation of mouse primary hepatocytes and describe quantification of β-fatty acid oxidation and lipogenesis.

 JoVE Biology

A Simplified System for Evaluating Cell Mechanosensing and Durotaxis In Vitro

1Department of Cell & Developmental Biology, SUNY Upstate Medical University


JoVE 52949

Many mammalian cells preferentially migrate towards a more rigid matrix or substrate through durotaxis. The goal of this protocol is to provide a simple in vitro system that can be used to study and manipulate cell durotaxis behaviors by incorporating polydimethylsiloxane (PDMS) substrates of defined rigidity, interfacing with glass coverslips.

 JoVE Biology

Using Caco-2 Cells to Study Lipid Transport by the Intestine

1Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, California Northstate University, 2Department of Biomedical Sciences, James H. Quillen College of Medicine, East Tennessee State University


JoVE 53086

Caco-2 cells can serve as an in vitro model to study the enterocyte transport of lipids, and lipid-soluble drugs/vitamins. The permeable membrane system separates the apical from the basolateral compartment, while the lentivirus expression system offers an effective gene overexpression method. The isolation of lipoproteins is confirmed by TEM.

 JoVE Biology

Production of Nurr-1 Specific Polyclonal Antibodies Free of Cross-reactivity Against Its Close Homologs, Nor1 and Nur77

1Molecular Neurobiology Laboratory, Psychiatry, McLean Hospital, 2Department of Biochemistry, College of Medicine, Konyang University


JoVE 52963

Here, we selectively target antibodies against a specific member of a highly conserved family of proteins by immunizing animals with their most divergent regions followed by removing cross reactive antibodies by pre-adsorption.

 JoVE Biology

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

1Cellular Networks Proteomics Unit, Laboratory of Systems Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health


JoVE 52959

This protocol describes how to perform absolute quantification assays of target proteins within complex biological samples using selected reaction monitoring. It was used to accurately quantify proteins of the mouse macrophage chemotaxis signaling pathway. Target peptide selection, assay development, and qualitative and quantitative assays are described in detail.

 JoVE Biology

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization

1Centre for Cancer Research, Westmead Millennium Institute for Medical Research and University of Sydney


JoVE 52840

This protocol describes the use of bromodeoxyuridine (BrdU) uptake to permit the temporal tracking of cells that were in S phase at a specific point in time. Addition of DNA dyes and antibody labeling facilitates detailed analysis of the fate of the S phase cells at later times.

 JoVE Biology

Silencing of BRCA2 to Identify Novel BRCA2-regulated Biological Functions in Cultured Human Cells

1Institute of Biomembranes and Bioenergetics, National Research Council


JoVE 52849

Gene silencing by siRNA represents a convenient experimental strategy to analyze BRCA2-dependent biological functions with immediate implications to better understand cancer biology. A method to efficiently silence BRCA2, along with the experimental procedure to detect and quantify changes in BRCA2 protein expression by immunoblotting in human cell lines, is presented.

 JoVE Biology

Surface Spreading and Immunostaining of Yeast Chromosomes

1Department of Radiation and Cellular Oncology, University of Chicago, 2Department of Molecular Genetics and Cell Biology, University of Chicago


JoVE 53081

A method for surface-spreading chromosomes from budding yeast is presented. This method is derived from a method previously described by Loidl and Klein. In addition, we demonstrate a procedure for immunostaining of spread chromosomes.

 JoVE Biology

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

1Department of Genetics, Stanford University, 2Stanford Center for Genomics and Personalized Medicine, Stanford University


JoVE 51872

Using protein microarrays containing nearly the entire S. cerevisiae proteome is probed for rapid unbiased interrogation of thousands of protein-protein interactions in parallel. This method can be utilized for protein-small molecule, posttranslational modification, and other assays in high-throughput.

 JoVE Biology

Physiology Lab Demonstration: Glomerular Filtration Rate in a Rat

1Tactical Combat Casualty Care Research, U.S. Army Institute of Surgical Research, 2Department of Pharmacology and Toxicology, Michigan State University, 3Southwest National Primate Research Center, Texas Biomedical Research Institute


JoVE 52425

The purpose of this protocol is to demonstrate the principles and techniques for measuring and calculating glomerular filtration rate, urine flow rate, and excretion of sodium and potassium in a rat. This demonstration can be used to provide students with an overall conceptual understanding of how to measure renal function.

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