Journal of Visualized Experiments

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Keyword Search: Neuroscience

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Video Articles

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This procedure describes a method for the isolation and culture of the murine organ of Corti with or without the spiral limbus and spiral ganglion neurons. We also demonstrate a method for the expression of an exogenous reporter gene in the organ of Corti explant by electroporation.
Launch Video

    Published: 02/04/2010

Mark Parker1, 2, 3
Aurore Brugeaud1, 2
Albert S. B. Edge1, 2, 4

1Department of Otology and Laryngology, Harvard Medical School

2Eaton-Peabody Laboratory, Massachusetts Eye and Ear Infirmary

3Department of Communication Sciences and Disorders, Emerson College

4Program in Speech and Hearing Bioscience and Technology, Division of Health Science and Technology, Harvard and MIT

 

Optokinetic response has been widely used to assess the visual functions of larval zebrafish. Nevertheless, the standard protocol for larval fish is not yet readily applicable in adults1-5. Here, we introduce how to measure the OKR of adult zebrafish using a new protocol which is established in our lab.
Launch Video

    Published: 02/02/2010

Su-Qi Zou
Wu Yin
Ming-Jing Zhang
Chun-Rui Hu
Yu-Bin Huang
Bing Hu

Laboratory of Neurodevelopment and Repair, Department of Neurobiology and Biophysics, School of Life, University of Science and Technology of China

 

Controlling and analyzing neural circuits in vivo would be facilitated by a technology for delivery of viruses and other reagents to desired 3-dimensional sets of brain regions. We demonstrate customized fluidic injector array fabrication, and delivery of virally-encoded optical sensitizers, enabling optical manipulation of complex brain circuits.
Launch Video

    Published: 01/21/2010

Stephanie Chan
Jacob Bernstein
Edward Boyden

Biological Engineering, Brain and Cognitive Sciences, and McGovern Institute, Massachusetts Institute of Technology

 

A demonstration of the isolation of neonatal mouse spinal cord for electrophysiologic studies.
Launch Video

    Published: 01/18/2010

Allyn Meyer1
Benjamin W. Gallarda1, 2
Samuel Pfaff1
William Alaynick1

1The Salk Institute for Biological Studies, Howard Hughes Medical Institute and Gene Expression Laboratory

2Biology Graduate Program, University of California, San Diego

 

Parkinson disease is caused by loss of dopaminergic innervation to the striatum, which can be experimentally induced by 6-OH-dopamine. We describe how to perform a stereotaxic lesion and to monitor apomorphine-induced rotational behavior in mice. This model is useful and reliable for testing new therapies for Parkinson disease.
Launch Video

    Published: 01/15/2010

Fabio S.L. da Conceição
Stacie Ngo-Abdalla
Jean-Christophe Houzel
Stevens K. Rehen

Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Brasil

 

This video article demonstrates the establishment of organotypic retinal wholemount cultures and a cytospin procedure for analysis of exogenously induced effects. Organotypic retinal wholemount cultures mimic the in vivo situation and significantly facilitate the accessibility of murine retinas for experimental manipulations while circumventing the disadvantages of classical murine animal models.
Launch Video

    Published: 01/11/2010

Sebastian Gustmann
Nicole Dünker

Institute for Anatomy, Department of Neuroanatomy, University of Duisburg-Essen, Essen, Germany

 

The endplate potential (EPP) component can be extracted from the surface EMG using a digital filter. The extracted EPP shows oscillation with a frequency of about 30 Hz.
Launch Video

    Published: 12/16/2009

Toshifumi Kumai

Graduate School of Oral Medicine, Matsumoto Dental University

 

Neurexins and neuroligins are membrane-neuron adhesion proteins which perform essential roles in synaptic differentiation and transmission. Neuroligin deficient mutants of C. elegans are defective in detecting osmotic strength, but when they also contain a mutation in the gene coding neurexin, they recover the wild type phenotype.
Launch Video

    Published: 12/11/2009

Fernando Calahorro1, 2
Encarna Alejandre1, 2
Manuel Ruiz-Rubio1, 2

1Departamento de Genética, Facultad de Ciencias, Universidad de Córdoba

2Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC)

 

Direct intranuclear injection of cDNA is an effective transfection technique for post-mitotic cells. This method provides high levels of heterologous protein expression from single or multiple cDNA constructs and enables protein function to be studied in a physiologically relevant environment with a variety of single cell assays.
Launch Video

    Published: 12/10/2009

Van B. Lu
Damian J. Williams
Yu-Jin Won
Stephen R. Ikeda

Laboratory of Molecular Physiology, National Institute on Alcohol Abuse and Alcoholism (NIAAA), National Institutes of Health (NIH)

 

A dissection technique for removal of the dentate gyrus from adult mouse under a stereomicroscope was demonstrated in this video-recorded protocol.
Launch Video

1 Comment    Published: 11/17/2009

Hideo Hagihara1, 2
Keiko Toyama1, 2
Nobuyuki Yamasaki2, 3
Tsuyoshi Miyakawa1, 2, 4, 5

1Division of Systems Medical Science, Institute for Comprehensive Medical Science, Fujita Health University

2Japan Science and Technology Agency, Core Research for Evolutionary Science and Technology (CREST)

3Department of Psychiatry, Kyoto University Graduate School of Medicine

4Genetic Engineering and Functional Genomics Group, Horizontal Medical Research Organization, Kyoto University Faculty of Medicine

5Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, National Institutes of Natural Sciences

 

 

Page:1 10 

 

Total: 133 articles.

 

Search results for 2/9/2010 6:22:22 AM

 

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Journal of Visualized Experiments (JoVE) is an online research journal employing visualization to increase reproducibility and transparency in biological sciences.

 

ISSN 1940-087X

 

 

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