JoVE Browse:Keyword Search: Neuroscience

Journal of Visualized Experiments

New Account

Forgot Password


		Search

Home

Browse

For Authors


			All	By Issue	By Category	By Keyword	Advanced Search

Keyword Search: Neuroscience

Page 1 2 3 4 5 6 7 8 9 10

Video Articles

Authors

Keywords

Primary Culture and Plasmid Electroporation of the Murine Organ of Corti.

This procedure describes a method for the isolation and culture of the murine organ of Corti with or without the spiral limbus and spiral ganglion neurons. We also demonstrate a method for the expression of an exogenous reporter gene in the organ of Corti explant by electroporation.

Launch Video

Published: 02/04/2010

Mark Parker^{1, 2, 3}
Aurore Brugeaud^{1, 2}
Albert S. B. Edge^{1, 2, 4}

¹Department of Otology and Laryngology, Harvard Medical School

²Eaton-Peabody Laboratory, Massachusetts Eye and Ear Infirmary

³Department of Communication Sciences and Disorders, Emerson College

⁴Program in Speech and Hearing Bioscience and Technology, Division of Health Science and Technology, Harvard and MIT

Neuroscience, Issue 36, hearing, mice, cochlea, organ of Corti, organotypic, culture, hair cell, stem cell, gene expression, in vitro

Using the optokinetic response to study visual function of zebrafish

Optokinetic response has been widely used to assess the visual functions of larval zebrafish. Nevertheless, the standard protocol for larval fish is not yet readily applicable in adults^1-5. Here, we introduce how to measure the OKR of adult zebrafish using a new protocol which is established in our lab.

Launch Video

Published: 02/02/2010

Su-Qi Zou
Wu Yin
Ming-Jing Zhang
Chun-Rui Hu
Yu-Bin Huang
Bing Hu

Laboratory of Neurodevelopment and Repair, Department of Neurobiology and Biophysics, School of Life, University of Science and Technology of China

Neuroscience, Issue 36, Zebrafish, OKR, OMR, behavior, optokinetic, vision

Scalable Fluidic Injector Arrays for Viral Targeting of Intact 3-D Brain Circuits

Controlling and analyzing neural circuits in vivo would be facilitated by a technology for delivery of viruses and other reagents to desired 3-dimensional sets of brain regions. We demonstrate customized fluidic injector array fabrication, and delivery of virally-encoded optical sensitizers, enabling optical manipulation of complex brain circuits.

Launch Video

Published: 01/21/2010

Stephanie Chan
Jacob Bernstein
Edward Boyden

Biological Engineering, Brain and Cognitive Sciences, and McGovern Institute, Massachusetts Institute of Technology

JoVE Neuroscience, Issue 35, Lentivirus, adeno-associated virus, channelrhodopsin, optogenetics, neuroscience, infusion, transgenic, gene therapy, drug delivery, neurotechnology, brain circuits

Spinal Cord Electrophysiology

A demonstration of the isolation of neonatal mouse spinal cord for electrophysiologic studies.

Launch Video

Published: 01/18/2010

Allyn Meyer¹
Benjamin W. Gallarda^{1, 2}
Samuel Pfaff¹
William Alaynick¹

¹The Salk Institute for Biological Studies, Howard Hughes Medical Institute and Gene Expression Laboratory

²Biology Graduate Program, University of California, San Diego

Neuroscience, Issue 35, Electrophysiology, central pattern generator, spinal cord, artificial cerebrospinal fluid

Murine Model for Parkinson's Disease: from 6-OH Dopamine Lesion to Behavioral Test

Parkinson disease is caused by loss of dopaminergic innervation to the striatum, which can be experimentally induced by 6-OH-dopamine. We describe how to perform a stereotaxic lesion and to monitor apomorphine-induced rotational behavior in mice. This model is useful and reliable for testing new therapies for Parkinson disease.

Launch Video

Published: 01/15/2010

Fabio S.L. da Conceição
Stacie Ngo-Abdalla
Jean-Christophe Houzel
Stevens K. Rehen

Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Brasil

Neuroscience, Issue 35, neurodegenerative disease, mice, cell therapy, model

In vivo-like Organotypic Murine Retinal Wholemount Culture

This video article demonstrates the establishment of organotypic retinal wholemount cultures and a cytospin procedure for analysis of exogenously induced effects. Organotypic retinal wholemount cultures mimic the in vivo situation and significantly facilitate the accessibility of murine retinas for experimental manipulations while circumventing the disadvantages of classical murine animal models.

Launch Video

Published: 01/11/2010

Sebastian Gustmann
Nicole Dünker

Institute for Anatomy, Department of Neuroanatomy, University of Duisburg-Essen, Essen, Germany

Neuroscience, Issue 35, eye, retina, organotypic culture, wholemount, dissociation, cytospin

Extraction of the EPP Component from the Surface EMG

The endplate potential (EPP) component can be extracted from the surface EMG using a digital filter. The extracted EPP shows oscillation with a frequency of about 30 Hz.

Launch Video

Published: 12/16/2009

Toshifumi Kumai

Graduate School of Oral Medicine, Matsumoto Dental University

Neuroscience, Issue 34, masseter muscle, EMG, EPP, neuromuscular junction, EPP oscillation

Osmotic Avoidance in Caenorhabditis elegans: Synaptic Function of Two Genes, Orthologues of Human NRXN1 and NLGN1, as Candidates for Autism

Neurexins and neuroligins are membrane-neuron adhesion proteins which perform essential roles in synaptic differentiation and transmission. Neuroligin deficient mutants of C. elegans are defective in detecting osmotic strength, but when they also contain a mutation in the gene coding neurexin, they recover the wild type phenotype.

Launch Video

Published: 12/11/2009

Fernando Calahorro^{1, 2}
Encarna Alejandre^{1, 2}
Manuel Ruiz-Rubio^{1, 2}

¹Departamento de Genética, Facultad de Ciencias, Universidad de Córdoba

²Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC)

Neuroscience, Microbiology, Issue 34, synapse, osmotic sensitivity, Caenorhabditis elegans, neurexin, neuroligin, autism, neuroscience

Intranuclear Microinjection of DNA into Dissociated Adult Mammalian Neurons

Direct intranuclear injection of cDNA is an effective transfection technique for post-mitotic cells. This method provides high levels of heterologous protein expression from single or multiple cDNA constructs and enables protein function to be studied in a physiologically relevant environment with a variety of single cell assays.

Launch Video

Published: 12/10/2009

Van B. Lu
Damian J. Williams
Yu-Jin Won
Stephen R. Ikeda

Laboratory of Molecular Physiology, National Institute on Alcohol Abuse and Alcoholism (NIAAA), National Institutes of Health (NIH)

Cellular Biology, Issue 34, Neuroscience, Molecular biology, Heterologous expression, Nuclear transfection, DNA injection, microinjection, SGC neurons, GIRK channels, neurons

Dissection of Hippocampal Dentate Gyrus from Adult Mouse

A dissection technique for removal of the dentate gyrus from adult mouse under a stereomicroscope was demonstrated in this video-recorded protocol.

Launch Video

1 Comment Published: 11/17/2009

Hideo Hagihara^{1, 2}
Keiko Toyama^{1, 2}
Nobuyuki Yamasaki^{2, 3}
Tsuyoshi Miyakawa^{1, 2, 4, 5}

¹Division of Systems Medical Science, Institute for Comprehensive Medical Science, Fujita Health University

²Japan Science and Technology Agency, Core Research for Evolutionary Science and Technology (CREST)

³Department of Psychiatry, Kyoto University Graduate School of Medicine

⁴Genetic Engineering and Functional Genomics Group, Horizontal Medical Research Organization, Kyoto University Faculty of Medicine

⁵Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, National Institutes of Natural Sciences

Neuroscience, Issue 33, Dentate gyrus, Hippocampus, dissection, neurogenesis, alpha-CaMKII, mouse

Page:1 2 3 4 5 6 7 8 9 10

Total: 133 articles.

Search results for 2/9/2010 6:22:22 AM

About Jove

Journal of Visualized Experiments (JoVE) is an online research journal employing visualization to increase reproducibility and transparency in biological sciences.

ISSN 1940-087X

Quick links

* Welcome

* Browse

* Submit

* About

* Press

* Contact