Phase Contrast and Differential Interference Contrast (DIC) Microscopy
Victoria Centonze Frohlich Core Optical Imaging Facility, University of Texas Health Science Center at San Antonio
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0:00 Title0 0:45 Introduction45 1:09 Phase Contrast69 3:14 Difference Interference Contrast Microscopy194 6:11 Conclusion371
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Phase-contrast microscopy is often used to produce contrast for transparent, non light-absorbing, biological specimens. The technique was discovered by Zernike, in 1942, who received the Nobel prize for his achievement. DIC microscopy, introduced in the late 1960s, has been popular in biomedical research because it highlights edges of specimen structural detail, provides high-resolution optical sections of thick specimens including tissue cells, eggs, and embryos and does not suffer from the phase halos typical of phase-contrast images. This protocol highlights the principles and practical applications of these microscopy techniques.
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Several video clips demonstrating phase contrast and DIC microscopy were taken from another video-article, entitled Layers of Symbiosis. JoVE graciously acknowledges this contribution made by the Leadbetter lab.
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Centonze Frohlich V (2008). Phase Contrast and Differential Interference Contrast (DIC) Microscopy. JoVE. 18. http://www.jove.com/index/details.stp?id=844, doi: 10.3791/844
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Allowed tags: i, b, u, sup, sub 
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| | 09/16/2008 11:15:39 AM
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jastromb responded with a statement of type: Neutral
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The systems vary widely to correlate with your needs. I would be happy to help you further. 631-547-8501, Ned Jastromb, Product Manager, Nikon Instruments, Inc.
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| | 09/18/2008 8:40:38 AM
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Dr. Una FitzGerald responded with a statement of type: Neutral
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Excellent - I'm definitely going to use this for my students, who are from a mixed background (biologists, physicists, chemists, engineers).
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| | 05/27/2009 7:42:08 AM
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Kisuto responded with a statement of type: Question
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I've seen that there we're colored images obtained using the DIC microscopy that looks like a 3-D image. Is there an approximation using DIC to measure the depth or height difference by just interpreting color differences?
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| | 05/27/2009 7:59:00 AM
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Kisuto responded with a statement of type: Question
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I've seen that there we're colored images obtained using the DIC microscopy that looks like a 3-D image. Is there an approximation using DIC to measure the depth or height difference by just interpreting color differences?
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Mailer.URL: http://www.jove.com/index/details.stp?id=844 |
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