Expansion of Human Peripheral Blood γδ T Cells using Zoledronate

JoVE 3182 9/09/2011

Makoto Kondo^1,2, Takamichi Izumi^1,2, Nao Fujieda^1,2, Atsushi Kondo^1,2, Takeharu Morishita^1,2, Hirokazu Matsushita¹, Kazuhiro Kakimi¹

¹Department of Immunotherapeutics (Medinet), University of Tokyo Hospital, ²MEDINET Co., Ltd

A method to expand γδ T cells from peripheral blood mononuclear cells (PBMC) is described. PBMC-derived γδ T cells are stimulated and expanded using zoledronate and interleukin-2 (IL-2). Large scale expansion of γδ T cells can be applied to autologous cellular immunotherapy of cancer.