Materials
Name | Company | Catalog Number | Comments |
B27 Supplement | Invitrogen | 17504-044 | |
Borax | Sigma | 71997 | |
Boric Acid | Sigma | B6768 | |
Bovine Serum Albumin | Sigma | A4503 | |
Fetal Bovine Serum | Invitrogen | 26140-079 | |
GlutaMAX | Invitrogen | 35050-061 | |
Glutamine | Invitrogen | 25030-081 | |
HBSS, no calcium, no magnesium, no phenol red | Invitrogen | 14175-095 | |
MEM, no glutamine, no phenol red | Invitrogen | 51200-038 | |
Neurobasal, no glutamine, no phenol red | Invitrogen | 12348-017 | |
Penicillin/Streptomycin | Invitrogen | 15070-063 | |
Poly-D-lysine, MW 70,000-150,000 | Sigma | P0899 | |
Trypan Blue 0.4% | Invitrogen | 15250-061 | |
EQUIPMENT | |||
Dumont #5/45 Angled Forceps | Fine Science Tools | 11251-35 | |
Dumont #7 – Fine Curved Forceps | Fine Science Tools | 11274-20 | |
Serrated (London) Forceps | Fine Science Tools | 11080-02 | |
Extra Fine Graefe Forceps 1 x 2 teeth | Fine Science Tools | 11153-10 | |
Moria Spring Scissors | Fine Science Tools | 15396-00 | |
Medium Operating Scissors | Roboz Surgical | RS-6812 | |
Extra Fine Microdissecting Scissors | Roboz Surgical | RS-5880 | |
24-well Tissue Culture Plates | Fisher | 87721 | |
Cell Strainer, 40 µM Nylon Mesh | BD Biosciences | 352340 |
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