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JoVE Journal Bioengineering
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Bioengineering

Merkmale der Niederschlag gebildet Polyethylene Glycol-Mikrogele durch Molekulargewicht der Reaktanden Controlled

Published: December 23, 2013 doi: 10.3791/51002
Automatic Translation
1, 1, 1,2, 1
1Department of Biomedical Engineering, The University of Akron, 2Saint Vincent Saint Mary's High School

Abstract

Diese Arbeit beschreibt die Bildung von Poly (ethylenglycol) (PEG)-Mikrogele über eine photopolymerisierte Fällungsreaktion. Fällungsreaktionen bieten mehrere Vorteile gegenüber herkömmlichen Mikrokügelchen Herstellungstechniken. Entgegen der Emulsion, Suspension, Dispersion und Techniken sind von gleicher Form und Größe Mikrogele durch Ausfällung gebildet, dh geringer Polydispersitätsindex ohne die Verwendung von organischen Lösemitteln oder Stabilisatoren. Die milden Bedingungen der Fällungsreaktion, anpassbare Eigenschaften der Mikrogele und niedrige Viskosität für Injektions machen sie gelten für in-vivo-Zwecke. Im Gegensatz zu anderen Herstellungstechniken können Mikrogel Eigenschaften durch Veränderung der Ausgangspolymermolekulargewicht geändert werden. Die Erhöhung der Start PEG-Molekulargewicht erhöht Mikrogel Durchmesser und Quellverhältnis. Weitere Modifikationen sind wie Kapselung Moleküle während Mikrogel Vernetzung vorgeschlagen. Einfache Anpassungen der PEG microgel Bausteine ​​sind für zukünftige Anwendungen der Mikrogele als Drug-Delivery-Fahrzeuge und Tissue Engineering Scaffolds erforscht.

Materials

Name Company Catalog Number Comments
Phosphate Buffered Saline (PBS) MP Biomedical 2810305
Triethanolamine (TEOA) J.T. Baker 9468-01 Preheat to 37 °C prior to pipetting
Hydrochloric acid (HCl) BDH Aristar BDH3028
Sodium Sulfate J.T. Baker 3891-01
Irgacure 2959 Ciba 029891301PS04
Ovalbumin (OVA) Invitrogen 34782
PEG 1,500 Alfa Aesar A16241
PEG 3,000 Fluka 03997-1KG
PEG 4,000 Alfa Aesar A16151
PEG 4,600 Sigma 373001-250G
PEG 6,000 Fluka 03394-1KG
PEG 10,000 Alfa Aesar B21955
Dextran 70 TCI D1449

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Merkmale der Niederschlag gebildet Polyethylene Glycol-Mikrogele durch Molekulargewicht der Reaktanden Controlled
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Cite this Article

Thompson, S., Stukel, J., AlNiemi,… More

Thompson, S., Stukel, J., AlNiemi, A., Willits, R. K. Characteristics of Precipitation-formed Polyethylene Glycol Microgels Are Controlled by Molecular Weight of Reactants. J. Vis. Exp. (82), e51002, doi:10.3791/51002 (2013).

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