Materials
Name | Company | Catalog Number | Comments |
Collagenase type II | Life Technologies | 17101-015 | |
Dispase | Life Technologies | 17105-041 | |
DMEM (1x) + GlutaMAX | Life Technologies | 31966-021 | |
Hank's Balanced Salt Solution | Life Technologies | 14025-050 | |
Canine Endothelial Cells Growth Medium | Cell Applications | Cn211-500 | |
CnAOECs | Cell Applications | Cn304-05 | |
Fetal Calf Serum (FCS) | GE Healthcare | 16000-044 | |
TrypLE Express | Life Technologies | 12604-013 | |
SPR | Bio-Rad | 170-8898 | |
iScript synthesis kit | Bio-Rad | 170-8891 | |
SYBR green super mix | Bio-Rad | 170-8886 | |
Recovery Cell Freezing Medium | Gibco/Life Technologies | 12648-010 | Keep on ice prior to use |
Freezing container, Nalgene Mr. Frosty | Sigma-Aldrich | C1562 | |
Gelatin | Sigma-Aldrich | G1890 | |
Surgical scissors (Mayo or Metzenbaum) | B. Braun Medical | BC555R | |
Mosquito forceps | B. Braun Medical | FB440R | |
Mosquito forceps curved | B. Braun Medical | FB441R | |
polyglactin 3-0 | Ethicon | VCP311H | |
Trypan blue | Bio-Rad | 145-0013 | |
Automated counting chamber | Bio-Rad | 145-0102 | |
Counting Slides, Dual Chamber | Bio-Rad | 145-0011 | |
Matrigel | BD Biosciences | BD356231 | Slowly thaw on ice |
µ-Slide Angiogenesis | Ibidi | 81501 | |
Endothelial Growth Medium | Lonza | CC-3156 | |
EGM-2 SingleQuot Kit | Lonza | CC-4176 |
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