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Medicine

Isolering og dyrkning af Primære endotelceller fra Canine arterier og vener

Published: November 18, 2016 doi: 10.3791/54786

Materials

Name Company Catalog Number Comments
Collagenase type II Life Technologies 17101-015
Dispase Life Technologies 17105-041
DMEM (1x) + GlutaMAX Life Technologies 31966-021
Hank's Balanced Salt Solution Life Technologies 14025-050
Canine Endothelial Cells Growth Medium  Cell Applications Cn211-500
CnAOECs Cell Applications Cn304-05
Fetal Calf Serum (FCS)  GE Healthcare 16000-044
TrypLE Express Life Technologies 12604-013
SPR Bio-Rad 170-8898
iScript synthesis kit Bio-Rad 170-8891
SYBR green super mix Bio-Rad 170-8886
Recovery Cell Freezing Medium Gibco/Life Technologies 12648-010 Keep on ice prior to use
Freezing container, Nalgene Mr. Frosty Sigma-Aldrich C1562
Gelatin Sigma-Aldrich G1890
Surgical scissors (Mayo or Metzenbaum) B. Braun Medical BC555R
Mosquito forceps  B. Braun Medical FB440R
Mosquito forceps curved B. Braun Medical FB441R
polyglactin 3-0 Ethicon VCP311H
Trypan blue Bio-Rad 145-0013
Automated counting chamber Bio-Rad 145-0102
Counting Slides, Dual Chamber Bio-Rad 145-0011
Matrigel BD Biosciences BD356231 Slowly thaw on ice
µ-Slide Angiogenesis Ibidi 81501
Endothelial Growth Medium Lonza CC-3156
EGM-2 SingleQuot Kit  Lonza CC-4176

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References

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  3. Murai, A., Asa, S. A., Kodama, A., Hirata, A., Yanai, T., Sakai, H. Constitutive phosphorylation of the mTORC2/Akt/4E-BP1 pathway in newly derived canine hemangiosarcoma cell lines. BMC Vet. Res. 8 (1), 128 (2012).
  4. Boilson, B. A., et al. Regulation of circulating progenitor cells in left ventricular dysfunction. Circ. Heart Fail. 3 (5), 635-642 (2010).
  5. Gonzalez-Miguel, J., Morchon, R., Siles-Lucas, M., Simon, F. Fibrinolysis and proliferative endarteritis: two related processes in chronic infections? The model of the blood-borne pathogen Dirofilaria immitis. PLoS One. 10 (4), e0124445 (2015).
  6. Sacks, T., Moldow, C. F., Craddock, P. R., Bowers, T. K., Jacob, H. S. Oxygen radicals mediate endothelial cell damage by complement-stimulated granulocytes. An in vitro model of immune vascular damage. J. Clin. Invest. 61 (5), 1161-1167 (1978).
  7. Aranguren, X. L., et al. Unraveling a novel transcription factor code determining the human arterial-specific endothelial cell signature. Blood. 122 (24), 3982-3992 (2013).
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  9. Crampton, S. P., Davis, J., Hughes, C. C. Isolation of human umbilical vein endothelial cells (HUVEC). J. Vis. Exp. (3), e183 (2007).
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  11. Brinkhof, B., Spee, B., Rothuizen, J., Penning, L. C. Development and evaluation of canine reference genes for accurate quantification of gene expression. Anal. Biochem. 356 (1), 36-43 (2006).
  12. Heishima, K., et al. MicroRNA-214 and MicroRNA-126 Are Potential Biomarkers for Malignant Endothelial Proliferative Diseases. Int. J. Mol. Sci. 16 (10), 25377-25391 (2015).
  13. Liu, M. M., Flanagan, T. C., Lu, C. C., French, A. T., Argyle, D. J., Corcoran, B. M. Culture and characterisation of canine mitral valve interstitial and endothelial cells. Vet. J. 204 (1), 32-39 (2015).
  14. van den Bossche, L., van Steenbeek, F. G. Canine congenital portosystemic shunts: disconnections dissected. The Veterinary Journal. 211, 14-20 (2015).
  15. Sobczynska-Rak, A., Polkowska, I., Silmanowicz, P. Elevated Vascular Endothelial Growth Factor (VEGF) levels in the blood serum of dogs with malignant neoplasms of the oral cavity. Acta Vet. Hung. 62 (3), 362-371 (2014).
  16. Zhang, Q., et al. In vitro and in vivo study of hydralazine, a potential anti-angiogenic agent. Eur. J. Pharmacol. 779, 138-146 (2016).
Isolering og dyrkning af Primære endotelceller fra Canine arterier og vener
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Cite this Article

Oosterhoff, L. A., Kruitwagen, H.More

Oosterhoff, L. A., Kruitwagen, H. S., Spee, B., van Steenbeek, F. G. Isolation and Culture of Primary Endothelial Cells from Canine Arteries and Veins. J. Vis. Exp. (117), e54786, doi:10.3791/54786 (2016).

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