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Chick ex ovo Culture and ex ovo CAM Assay: How it Really Works

Article DOI: 10.3791/1620-v • 17:23 min • November 30th, 2009
November 30th, 2009 •
1, 1, 2, 3, 4, 1, 2
1Institute for Physiological Chemistry, Department of Biochemical Endocrinology, University of Duisburg-Essen, 2Institute for Anatomy, Department of Neuroanatomy, University of Duisburg-Essen, 3Morphoplant GmbH, 4ARCONS Institute for Applied Research and Didactics

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Summary
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The chick chorioallantoic membrane (CAM) is a unique, naturally immunodeficient supportive culture environment to study angiogenesis and tumorigenesis. This video article demonstrates the different steps in chick ex ovo culture, application of potentially angiogenic substances and successful inoculation of tumor cells and tissues on the surface of the CAM.

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Chick Ex Ovo Culture Ex Ovo CAM Assay Chicken Eggs Angiogenesis Tumorigenesis Chick Chorioallantoic Membrane CAM Blood Vessel Network Immunodeficient Host Grafting Tissues And Cells Tumor Cell Intravasation Micro Metastatic Foci Experimental Limitations Shell-less Cultures Survival Rates
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