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Live Cell Response to Mechanical Stimulation Studied by Integrated Optical and Atomic Force Microscopy

Article DOI: 10.3791/2072-v • 09:20 min • October 4th, 2010
October 4th, 2010 •
1,2, 1
1Department of Systems Biology and Translational Medicine, College of Medicine, Cardiovascular Research Institute, Texas A&M Health Science Center, 2Department of Biomedical Engineering, Texas A&M University

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Summary
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This paper aims to instruct the reader in the operation of an integrated atomic force-optical imaging microscope for mechanical stimulation of live cells in culture. A step-by-step protocol is presented. A representative data set that shows live cell response to mechanical stimulation is presented.

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Live Cell Response Mechanical Stimulation Integrated Optical Microscopy Atomic Force Microscopy Sub-cellular Level Spatial Resolution Temporal Resolution Total Internal Reflection Fluorescence Microscopy Fast-spinning Disk Confocal Microscopy Molecular Dynamic Studies Adherent Live Cells Optical Imaging Real-time Actin Filaments Focal Adhesions Cellular Structure Cell Restructuring Dynamics Detailed Protocol
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