JoVE   
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  JoVE General

  
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  JoVE Neuroscience

  
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  JoVE Immunology and Infection

  
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  JoVE Clinical and Translational Medicine

  
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  JoVE Bioengineering

  
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  JoVE Applied Physics

  
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  JoVE Chemistry

  
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  JoVE Behavior

  
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  JoVE Environment

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JoVE Science Education

General Laboratory Techniques

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Basic Methods in Cellular and Molecular Biology

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Model Organisms I

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Model Organisms II

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Institution Web Site

Texas A&M University

18 articles published in JoVE

 JoVE Immunology and Infection

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays

1Plant Pathology and Microbiology, Texas A&M University


JoVE 3727

The devastation of cereal crops by seed-infecting fungi has prompted numerous research efforts to better understand plant-pathogen interactions. To study seed-fungal interactions in a laboratory setting, we developed a robust method for the quantification of fungal reproduction, biomass, and mycotoxin contamination using kernel bioassays.

 JoVE Bioengineering

Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy

1Department of Computer Science and Engineering, Texas A&M University, 2Beckman Institute for Advanced Science and Technology, University of Illinois, 3Department of Electrical and Computer Engineering, Kettering University, 43Scan, 5Department of Veterinary Integrative Biosciences, Texas A&M University


JoVE 3248

The full process from brain specimen preparation to serial sectioning imaging using the Knife-Edge Scanning Microscope, to data visualization and analysis is described. This technique is currently used to acquire mouse brain data, but it is applicable to other organs, other species.

 JoVE General

Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton

1Department of Biochemistry and Biophysics, Institute of Plant Genomics and Biotechnology, Texas A&M University, 2Department of Plant Pathology and Microbiology, Institute of Plant Genomics and Biotechnology, Texas A&M University


JoVE 2938

We present the detailed protocol for Agrobacterium-mediated virus-induced gene silencing (VIGS) assay in cotton. The tobacco rattle virus (TRV)-derived VIGS vectors were deployed to induce RNA silencing of cotton GrCLA1, Cloroplastos alterados 1 gene. The albino phenotype caused by silencing GrCLA1 was observed at the seedling stage within 2 weeks after inoculation.

 JoVE General

A β-glucuronidase (GUS) Based Cell Death Assay

1Department of Plant Pathology and Microbiology, Institute for Plant Genomics and Biotechnology, Texas A&M University


JoVE 2680

Programmed cell death assays commonly used in mammalian systems such as DNA laddering or TUNEL assays, are often difficult to reproduce in plants. In combination with a GUS reporter system, we propose a rapid, plant based transient assay to analyze the potential death properties of specific genes.

 JoVE Immunology and Infection

A Calcium Bioluminescence Assay for Functional Analysis of Mosquito (Aedes aegypti) and Tick (Rhipicephalus microplus) G Protein-coupled Receptors

1Department of Entomology, Texas A&M University (TAMU), 2Department of Molecular and Cellular Medicine, Texas A&M University (TAMU)


JoVE 2732

This protocol provides instructions for clonal-cell line selection and a calcium bioluminescence assay to analyze the structure-activity relationships of synthesized arthropod neuropeptides on their cognate GPCRs. This assay can be used for receptor deorphanization and structure-activity relationship studies for synthetic analog design and peptide/drug-lead discovery.

 JoVE General

Rapid PCR Thermocycling using Microscale Thermal Convection

1Department of Mechanical Engineering, Texas A&M University, 2Department of Mechanical Engineering and Department of Nuclear Engineering, Texas A&M University, 3Department of Chemical Engineering, Texas A&M University


JoVE 2366

We describe a novel method to perform DNA replication via the polymerase chain reaction (PCR). Thermal convection is harnessed to continuously shuttle reagents between denaturing, annealing, and extension conditions by maintaining opposing surfaces of the reactor at constant temperature. This inherently simple design promises to make rapid PCR more accessible.

 JoVE Immunology and Infection

Using Luciferase to Image Bacterial Infections in Mice

1Microbial & Molecular Pathogenesis, Texas A&M Health Science Center


JoVE 2547

Methods for bioluminescence imaging of bacterial infections in living animals are decribed. Pathogens are modified to express luciferase allowing optical whole body imaging of infections in live animals. Animal models can be infected with luciferase expressing pathogens and the resulting course of disease visualized in real-time by bioluminescence imaging.

 JoVE Neuroscience

Automated Interactive Video Playback for Studies of Animal Communication

1Department of Visualization, Texas A&M University (TAMU), 2Department of Biology, Texas A&M University (TAMU)


JoVE 2374

Video playback is a widely used technique in animal behavior. We created and evaluated a program that applies rules-based, interactive playback of 3-D computer animations in response to real-time, automated data on subject behavior.

 JoVE General

Live Cell Response to Mechanical Stimulation Studied by Integrated Optical and Atomic Force Microscopy

1Department of Systems Biology and Translational Medicine, College of Medicine, Cardiovascular Research Institute, Texas A&M Health Science Center, 2Department of Biomedical Engineering, Texas A&M University


JoVE 2072

This paper aims to instruct the reader in the operation of an integrated atomic force-optical imaging microscope for mechanical stimulation of live cells in culture. A step-by-step protocol is presented. A representative data set that shows live cell response to mechanical stimulation is presented.

 JoVE General

Microfluidic Co-culture of Epithelial Cells and Bacteria for Investigating Soluble Signal-mediated Interactions

1McFerrin Department of Chemical Engineering, Texas A&M University, 2Department of Biomedical Engineering, Texas A&M University


JoVE 1749

This protocol describes a microfluidic co-culture model for simultaneous and localized culture of epithelial cells and bacteria. This model can be used for investigating the role of different soluble molecular signals on pathogenesis as well as screen the effectiveness of putative probiotic bacterial strains.

 JoVE General

A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients

1McFerrin Department of Chemical Engineering, Texas A&M University, 2Department of Biology, Texas A&M University, 3Department of Biomedical Engineering, Texas A&M University


JoVE 1779

This protocol describes the development of a microfluidic device for investigating bacterial chemotaxis in stable concentration gradients of chemoeffectors.

 JoVE General

Preparation of Rat Brain Aggregate Cultures for Neuron and Glia Development Studies

1Department of Veterinary Integrative Biosciences, Texas A&M University (TAMU)


JoVE 1304

A protocols for an embryonic rat brain aggregate culture system is described. Multipotent progenitors in the aggregates can develop and differentiate into neurons, astrocytes and oligodendrocytes.

 JoVE General

A Multi-compartment CNS Neuron-glia Co-culture Microfluidic Platform

1Department of Electrical and Computer Engineering, Texas A&M University (TAMU), 2Department of Veterinary Integrative Biosciences, Texas A&M University (TAMU)


JoVE 1399

We developed a novel multi-compartment neuron co-culture microsystem platform for in vitro CNS axon-glia interaction research. The platform is capable of conducting up to six independent experiments in parallel and was fabricated using a newly developed macro/micro hybrid fabrication method.

 JoVE General

Antifouling Self-assembled Monolayers on Microelectrodes for Patterning Biomolecules

1Department of Physics, Texas A&M University (TAMU), 2Department of Biomedical Engineering, Texas A&M University (TAMU)


JoVE 1390

We present a procedure for forming a poly(ethylene glycol) self-assembled monolayer (PEG-SAM) on a silicon substrate with gold microelectrodes. The PEG-SAM is formed in a single step and prevents biofouling on silicon and gold surfaces. Electrophoresis is then used for patterning biomolecules down to the nanoscale.

 JoVE General

Assay for Neural Induction in the Chick Embryo

1Institute of Biosciences and Technology, Center for Environmental and Genetic Medicine, Texas A&M University (TAMU)


JoVE 1027

Neural induction is the first step in the formation of the brain. It is a mechanism by which Hensen's node (organizer), instructs adjacent tissue to adopt a neural fate, i.e. to give rise to the nervous system. This video demonstrates an assay for neural induction in chick embryo.

 JoVE General

Method for Whole Mount Antibody Staining in Chick

1Institute of Biosciences and Technology, Center for Environmental and Genetic Medicine, Texas A&M University (TAMU)


JoVE 956

This video demonstrates whole mount immunohistochemistry, a method by which the spatial and temporal expression pattern of an antigen can be visualized in young chick embryos. This method was originally introduced by Jane Dodd and Tom Jessell.

 JoVE General

Double Whole Mount in situ Hybridization of Early Chick Embryos

1Center for Environmental and Genetic Medicine, Institute of Biosciences and Technology - Texas A&M Health Science Center, 2Center for Environmental and Genetic Medicine, Texas A&M University (TAMU)


JoVE 904

This video demonstrates 2-color whole mount in situ hybridization, a method by which the spatial and temporal expression pattern of 2 different genes can be visualized in young chick embryos. This method was originally introduced by David Wilkinson, Domingos Henrique, Phil Ingham and David Ish -Horowicz.

 JoVE General

Method for Culture of Early Chick Embryos ex vivo (New Culture)

1Center for Environmental and Genetic Medicine, Institute of Biosciences and Technology - Texas A&M Health Science Center, 2Center for Environmental and Genetic Medicine, Texas A&M University (TAMU)


JoVE 903

This video demonstrates New culture, a method by which chick embryos are cultured outside the egg for up to 24 hr. This method enables one to study early development (primitive streak to 14 som.), a period corresponding to E7-9 in mouse. Applications of this technique include electroporation, in situ hybridization and immunohistochemistry.

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