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Biology

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Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells
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Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells

Article DOI: 10.3791/3110-v • 10:25 min • October 5th, 2011
October 5th, 2011 •
1, 1, 1
1UCR Stem Cell Center, Department of Cell Biology and Neuroscience, University of California Riverside

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Despite recent advancements in genetic modification, transfection of human embryonic stem cells (HESCs) remains a capricious process. To our knowledge, systematic and efficient methods to transfect human induced pluripotent stem cells (iPSCs) have not been reported. Here, we describe robust protocols to efficiently transfect and nucleofect human iPSCs.

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Transfecting Nucleofecting Induced Pluripotent Stem Cells Genetic Modification Stem Cell Biology Clinical Applications Gene Delivery Methods HESCs Human Embryonic Stem Cells Human Induced Pluripotent Stem Cells IPSCs Plasmid Enhanced Green Fluorescence Protein EGFP Reporter Feeder-free Cultures Feeder Cell Transfection Stable Transgenic IPSC Clones ROCK Inhibitor Trypsinized Cells Feeders Feeder Cell-conditioned Medium Transgene-expressing IPSCs Antibiotic Selection Stable Transgenic Lines Pluripotency
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