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Journal / Bioengineering / Spatio-tidsmæssige Manipulation af Lille GTPase…
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Bioengineering

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JoVE Journal Bioengineering
Spatio-tidsmæssige Manipulation af Lille GTPase aktivitet på Subcellulær niveau og på Tidsplan for Seconds i levende celler
Quantification of Global Diastolic Function by Kinematic Modeling-based Analysis of Transmitral Flow via the Parametrized Diastolic Filling Formalism
Journal (Bioengineering)
Quantification of Global Diastolic Function by Kinematic Modeling-based Analysis of Transmitral Flow via the Parametrized Diastolic Filling Formalism
Thermodynamics of Membrane Protein Folding Measured by Fluorescence Spectroscopy
Journal (Bioengineering)
Thermodynamics of Membrane Protein Folding Measured by Fluorescence Spectroscopy
 
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Spatio-tidsmæssige Manipulation af Lille GTPase aktivitet på Subcellulær niveau og på Tidsplan for Seconds i levende celler

Article DOI: 10.3791/3794-v • 10:28 min • March 9th, 2012
March 9th, 2012 •
1, 1, 1,2, 1,2, 2, 1,3, 1
1Department of Cell Biology, Center for Cell Dynamics, Johns Hopkins University, 2Graduate School of Pharmaceutical Sciences, University of Tokyo, 3Biomedical Engineering, Johns Hopkins University

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En fremgangsmåde til spatio-temporal kontrol lille GTPase-aktivitet af lys, er beskrevet. Denne metode er baseret på rapamycin-induceret FKBP-FRB heterodimerisering og foto-bure systemer. Optimering af lys-bestråling muliggør spatio-tidsmæssigt styret aktivering af små GTPaser på det subcellulære niveau.

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Bioengineering Lille GTPase rapamycin bur stof spatiotemporal kontrol heterodimerisering FKBP FRB lys bestråling
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