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JoVE Journal
Neuroscience

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代的永生化小鼠脑微血管内皮细胞株作为
 
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代的永生化小鼠脑微血管内皮细胞株作为

Article DOI: 10.3791/4022-v 09:37 min August 29th, 2012
August 29th, 2012

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Summary

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此方法描述了如何从小鼠脑微血管内皮细胞隔离和不朽。我们描述了一个一步一步的协议,从同质化的脑组织,消化步骤,播种和永生化的细胞。通常情况下,大约需要五周获得一个同质的,永生的微血管内皮细胞系。

Tags

第66期,免疫学,神经科学,血 - 脑屏障,
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