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Hög genomströmning Rening av Affinity-märkta rekombinanta proteiner
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Hög genomströmning Rening av Affinity-märkta rekombinanta proteiner

Article DOI: 10.3791/4110-v • 07:45 min • August 26th, 2012
August 26th, 2012 •
1, 1
1Department of Life Sciences, Imperial College London

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Vi beskriver ett förfarande för affinitets-märkta rening av rekombinanta proteiner med användning av vätske-hantering robotteknik. Denna metod är allmänt tillämpbar på den småskaliga rening av lösliga His-märkta proteiner i en hög genomströmning formatet.

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Biokemi genetik molekylärbiologi bioinformatik rekombinanta proteiner histidin tag affinitetsrening hög genomströmning automation
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