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Journal / Biology / Enda molekyl avbildning av genreglering
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Enda molekyl avbildning av genreglering
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Enda molekyl avbildning av genreglering

Article DOI: 10.3791/50042-v • 11:31 min • March 15th, 2013
March 15th, 2013 •
1, 1,2,3, 1
1Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, 2Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 3Department of Physics, Jilin University

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Vi beskriver en fluorescensmikroskopi metod, Co-translationell aktivering genom klyvning (CoTrAC), att avbilda produktionen av proteinmolekyler i levande celler med en enda molekyl precision utan att störa proteinets funktionalitet. Denna metod har använts för att följa de stokastiska expressions dynamiken i en transkriptionsfaktor, den λ repressorn CI

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Biofysik 73 biokemi genetik kemi molekylärbiologi cellbiologi mikrobiologi proteiner enda molekyl fluorescerande protein protein uttryck cotranslational aktivering CoTrAC cellodling fluorescensmikroskopi bildbehandling translationell aktivering systembiologi
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