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Journal / Biology / Giant Liposome Forberedelse til Imaging and…
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Giant Liposome Forberedelse til Imaging and Patch-Clamp Electrophysiology
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Giant Liposome Forberedelse til Imaging and Patch-Clamp Electrophysiology

Article DOI: 10.3791/50227-v • 09:03 min • June 21st, 2013
June 21st, 2013 •
1, 1
1Department of Physiology and Biophysics, University of Washington

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Reconstituting funksjonelle membran proteiner i gigantiske liposomer definert sammensetning er en kraftig tilnærming når det kombineres med patch-clamp elektrofysiologi. Imidlertid kan konvensjonell gigantisk liposomen produksjonen være uforenlig med protein stabilitet. Vi beskriver protokoller for å produsere gigantiske liposomer fra rene lipider eller små liposomer som inneholder ionekanaler.

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Fysiologi biofysikk molekylærbiologi biokjemi genetikk cellebiologi Proteiner membraner Kunstige lipid bilayers Liposomes Fosfolipider biokjemi lipider Giant unilamillære Blemmer liposomet elektrofysiologi electroformation tilberedning patch clamp
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