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JoVE Journal
Biology

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一个协议,用于噬菌体展示和亲和选择以重组蛋白诱饵
 
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一个协议,用于噬菌体展示和亲和选择以重组蛋白诱饵

Article DOI: 10.3791/50685-v 12:36 min February 16th, 2014
February 16th, 2014

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

噬菌体展示是一种强大的技术,以捕获与感兴趣的固定化分子相互作用的蛋白质或蛋白质的部分。一旦噬菌体cDNA文库的类型来创建和屏幕的决定已经作出,这里所描述的协议允许高效亲和选择,导致识别干扰作用的。

Tags

生物化学,84期,亲和选择,噬菌体展示,蛋白 - 蛋白相互作用
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