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Journal / Neuroscience / Calciumphosphat Transfektion af primær…
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Neuroscience

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JoVE Journal Neuroscience
Calciumphosphat Transfektion af primær hippocampusneuroner
Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY
Journal (Neuroscience)
Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY
Single-Cell Electroporation across Different Organotypic Slice Culture of Mouse Hippocampal Excitatory and Class-Specific Inhibitory Neurons
Journal (Neuroscience)
Single-Cell Electroporation across Different Organotypic Slice Culture of Mouse Hippocampal Excitatory and Class-Specific Inhibitory Neurons
 
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Calciumphosphat Transfektion af primær hippocampusneuroner

Article DOI: 10.3791/50808-v • 08:22 min • November 12th, 2013
November 12th, 2013 •
*1, *1, 1, 1, 1
1Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, Rutgers University
* These authors contributed equally

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Calciumphosphatpræcipitation er en praktisk og økonomisk fremgangsmåde til transfektion af dyrkede celler. Med optimering, er det muligt at anvende denne metode på svære at transficere celler som primære neuroner. Her beskriver vi vores detaljerede protokol for caiciumphosphattransfektion af hippocampus neuroner dyrket sammen med astrogliale celler.

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Neuroscience Primær hippocampus neuron calciumphosphat-transfektion cokultur astrogliale celler DNA
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