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JoVE Journal Biology
Gist luminometrische en
Fabrication and Use of MicroEnvironment microArrays (MEArrays)
Journal (Biology)
Fabrication and Use of MicroEnvironment microArrays (MEArrays)
Clonogenic Assay: Adherent Cells
Journal (Biology)
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Gist luminometrische en

Article DOI: 10.3791/50816-v • 12:09 min • December 31st, 2013
December 31st, 2013 •
1, 1, 1, 1,2
1Laboratory of Cell and Molecular Biology, University of Wisconsin – Madison, 2Department of Genetics, University of Wisconsin – Madison

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Ter aanvulling gebruikte methoden bestuderen TRPV4's worden twee methoden beschreven: De mechanische gevoeligheid kan worden bestudeerd door Ca 2 +-aequorine luminometrie in transgene gist bij hypo-osmotische uitdaging. Het kan ook in TRPV4-RNA worden onderzocht geïnjecteerd Xenopus oöcyten door whole-cell twee-elektrode voltage clamp of patch klem in op cel-of uitgeschakelde modus.

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Basis Protocol Eukaryota Archaea Bacteriën Life Sciences (Algemeen) Mechanosensation Ionkanalen lipiden patch clamp, gist luminometrie kracht sensing voltage clamp TRPV4 elektrofysiologie
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