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Diavoorbereidingsmethode om driedimensionale chromatinearchitectuur van testiculaire kiemcellen te behouden
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Diavoorbereidingsmethode om driedimensionale chromatinearchitectuur van testiculaire kiemcellen te behouden

Article DOI: 10.3791/50819-v • 07:34 min • January 10th, 2014
January 10th, 2014 •
1,2
1Division of Reproductive Sciences, Division of Developmental Biology, Perinatal Institute, Cincinnati Children's Hospital Medical Center, 2Department of Pediatrics, University of Cincinnati College of Medicine

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Het materiaal hier beschrijft een methode die is ontwikkeld om de driedimensionale chromatinestructuur van testiculaire kiemcellen te behouden. Dit wordt de driedimensionale (3D) diamethode genoemd. Deze methode verbetert de gevoeligheid voor detectie van subnucleaire structuren en is toepasbaar voor immunofluorescentie, DNA en RNA fluorescentie in situ hybridisatie (FISH).

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Basisprotocol Chromatine Kiemcellen Geslachtschromosomen Testis Meiotische geslachtschromosoominactivatie Postmeiotische sekschromtine
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