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JoVE Journal
Biology

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芽殖酵母蛋白质的提取和纯化功能研究,交互和翻译后修饰
 
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芽殖酵母蛋白质的提取和纯化功能研究,交互和翻译后修饰

Article DOI: 10.3791/50921-v 09:22 min October 30th, 2013
October 30th, 2013

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Summary

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高品质的酵母细胞提取物的制备是一个必要的分析单个蛋白质或整个蛋白质组的第一步。在这里,我们描述了一个快速,高效,可靠的同质化协议已被优化,以保持蛋白质的功能,相互作用和翻译后修饰的芽殖酵母细胞。

Tags

基本议定书“,第80期,生命科学(综合),芽殖酵母,蛋白提取物,珠跳动,相扑,泛素,翻译后修饰,融合蛋白的亲和标签
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