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JoVE Journal Biology
Dissezione dei
Quantification of Intramuscular Adipose Tissue (IMAT) Lipid Droplet Metrics Using Confocal Imaging (Video) | JoVE
Journal (Biology)
Quantification of Intramuscular Adipose Tissue (IMAT) Lipid Droplet Metrics Using Confocal Imaging (Video) | JoVE
Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis
Journal (Biology)
Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis
 
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Dissezione dei

Article DOI: 10.3791/51118-v • 09:07 min • March 4th, 2014
March 4th, 2014 •
1,2,3,4, 1,2,3,4
1Institut Curie, Centre Universitaire, 2Université Paris Sud, Centre Universitaire, 3CNRS UMR 3347, Centre Universitaire, 4INSERM U1021, Centre Universitaire

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Questo protocollo descrive come sezionare premigratory cresta neurale cranica (NC) da Xenopus laevis neurulas. Tali espianti possono essere placcati in fibronectina e coltivate in vitro, o innestate di nuovo in embrioni di accoglienza. Questa tecnica consente di studiare i meccanismi di NC epitelio a mesenchima transizione, migrazione e differenziazione.

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Biologia dello Sviluppo Numero 85 Neural cresta, Embrione dissezione innesto fibronectina
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