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JoVE Journal
Chemistry

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通过超性能液相色谱对细菌细胞壁进行隔离和制备,进行组成分析
 
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通过超性能液相色谱对细菌细胞壁进行隔离和制备,进行组成分析

Article DOI: 10.3791/51183-v 11:18 min January 15th, 2014
January 15th, 2014

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Summary

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细菌细胞壁由多肽组成,多肽交叉连接的糖链大分子网络。超性能液态色谱为多肽类成分的新发现提供了高分辨率和高吞吐量。我们提出了一个隔离细胞壁(sacculi)的程序,以及随后通过 UPLC 进行分析的准备。

Tags

化学,第83期,多肽,细菌细胞壁,超性能液相色谱,高性能液相色谱,细胞形状,形态形成
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