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Nanopodia - 薄，易碎膜预测与细胞运动和细胞间的相互作用角色
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Nanopodia - 薄，易碎膜预测与细胞运动和细胞间的相互作用角色

Article DOI: 10.3791/51320-v • 10:50 min • April 3rd, 2014
April 3rd, 2014 •
1, 1, 1, 1
1Center for Vascular Biology Research, Department of of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School

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Nanopodia很瘦但很脆弱的膜通道，向​​上延伸至100微米的细胞的领先的前面或后面尾随和检测的细胞环境。直接固定在较高的Triton X-100浓度为37℃，轻柔洗涤，并避免如乙醇，甲醇，丙酮或有机溶剂，并须遵守这些细胞结构。

Tags

细胞生物学，第86期，nanopodia，TM4SF1，内皮细胞，肿瘤细胞，F-肌动蛋白，免疫荧光染色，四旋
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