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Biolistisk Transformation av en fluorescerande taggade gen i Opportunistic svamppatogen

Article DOI: 10.3791/52666-v • 07:32 min • March 19th, 2015
March 19th, 2015 •
1, 2, 1, 1
1Department of Genetics and Biochemistry, Eukaryotic Pathogens Innovation Center (EPIC), Clemson University, 2Department of Biology, Western Carolina University

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Biolistisk omvandling är en metod som används för att generera en stabil integrering av DNA i genomet hos de opportunistiska patogenen Cryptococcus neoformans genom homolog rekombination. Vi kommer att visa biolistisk transformation av en konstruktion, som har den gen som kodar acetatkinas smält till den fluorescerande taggen mCherry in C. neoformans.

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Molecular Biology genavbrott, Biolistisk leverans acetatkinas överlappning PCR fluorescens
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